Carcinogenesis, Teratogenesis & Mutagenesis ›› 2010, Vol. 22 ›› Issue (5): 356-360.doi: 10.3969/j.issn.1004-616x.2010.05.007

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Effects of thioglycolic acid on parthenogenetic activation of xenopus oocytes

REN Xiao-mei1， XIA Lei2， WANG Dong1， ZHANG Ling1，HOU Shao-ying1， ZHAO Wei3， WU Kun1,   

  1. 1. Department of Nutrition and Food Hygiene, School of Public Health, Harbin Medical University, Harbin 150081;2. Department of Reproductive Medicine, The First Affiliated Hospital of Harbin Medical University, Harbin 150001; 3.The Second Department of Orthopaedics,the First Hospital in Harbin,Harbin 150010, China
  • Received:2010-05-28 Revised:2010-06-30 Online:2010-09-30 Published:2010-09-30
  • Contact: WU Kun

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Abstract: OBJECTIVE: To investigate the effects of thioglycolic acid(TGA) on calcium ionophore A23187_ induced parthenogenetic activation of xenopus oocytes and its possible mechanism. METHODS: Xenopus oocytes were pretreated with TGA in vitro at the doses of 0, 5, 25, 125 μg/ml, and then activated by A23187. During this process, the activation rates were noted. Oocytes were collected 1h after the addition of A23187 and stained with Hoechst 33258 to check the formation of pronucleus. RESULTS: Following TGA treatment, the black spot appeared on the animal pole, known as fertilization coat, which was not in good condition. TGA significantly inhibited the rate of parthenogenetic activation(P<0.05) and the formation of pronucleus. Through Western Blotting analysis, TGA inhibited the degradation of Mos, p_ERK1 and CyclinB2. CONCLUSION:TGA treatment could inhibit the parthenogenetic activation of xenopus oocytes induced by A23187, possibly due to the down_regulation of MPF and MAPK degradation.

Key words: thioglycolic acid, oocytes, parthenogenetic activation