Carcinogenesis, Teratogenesis & Mutagenesis ›› 2010, Vol. 22 ›› Issue (5): 366-369.doi: 10.3969/j.issn.1004-616x.2010.05.009

Previous Articles     Next Articles

PIN1 gene amplification and overexpression in human laryngeal squamous cell carcinoma

LI Wei1，2, LI Hai-zhou2, LI Fu-cai1,   

  1. 1. Department of Medical Genetics, China Medical University, Shenyang 110001, Liaoning; 2. Jinzhou Central Hospital, Jinzhou 121000, Liaoning, China
  • Received:2010-01-18 Revised:2010-06-06 Online:2010-09-30 Published:2010-09-30
  • Contact: LI Fu-cai

PDF (PC)

1470

Abstract: 　　【ABSTRACT】 OBJECTIVE: To investigate the amplification and overexpression of PIN1 gene in human laryngeal squamous cell carcinoma. METHODS: PIN1 gene amplification(increase of gene copy number) was detected by differential PCR and Karyotype analysis. PIN1 mRNA was evaluated by RT_PCR. Furthermore, immunohistochemistry and Western Blot were employed to detect the PIN1 protein. RESULTS: Compared with nomal control, PIN1 gene amplification rate was 22.5％(9/40), while 17.65％(3/17) was found to have an increase of chromosome 19; the rate of mRNA overexpression was 67.5％(27/40). PIN1 protein was obviously upregulated, the ratio was 65％(26/40)(P<0.05). CONCLUSION: PIN1 gene copy increased, with mRNA and protein overexpression in laryngeal carcinoma. This fact may be relevant to laryngeal cancer development.

Key words: laryngeal squamous cell carcinoma, PIN1 gene, gene amplification, overexpression