Carcinogenesis, Teratogenesis & Mutagenesis ›› 2010, Vol. 22 ›› Issue (5): 383-385.doi: 10.3969/j.issn.1004-616x.2010.05.013

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Clinical analysis of preimplantation genetic diagnosis with fluorescence in situ hybridization

LIU Kun, ZHANG Xue_hong, REN Yu-hong, ZHAO Li-hui, SHI Xin, XUE Shi-long, MA Xiao-ling, JIA Xue-ling   

  1. Reproductive Medical Center of the First Hospital of Lanzhou University, Lanzhou 730000, China
  • Received:2009-12-14 Revised:2010-04-22 Online:2010-09-30 Published:2010-09-30
  • Contact: ZHANG Xue-hong

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Abstract: OBJECTIVE: To evaluate clinical significance of application of fluorescence in situ hybridization in preimplantation genetic diagnosis (PGD) for carriers of chromosomal abnormalities. METHODS: according to the categories of chromosomal abnormalities, we selected the appropriate sub_telomeric probes and centromere probes, or sex chromosome probe, hybridized once or twice so the 7 carriers received preimplantation genetic diagnosis. RESULTS: Preimplantation genetic diagnosis were performed in 7 cycles of 7 couples. A total of 131 oocytes were retrived, 77 embryos were available for biopsy, 87 blastomeres detected, 20 embryos transplanted, 4 clinical pregnancies obtained, and 2 healthy babies had been. CONCLUSION: Application of fluorescence in situ hybridization technique in preimplantation embryo genetic diagnosis for carriers of chromosomal abnormalities was an effective way.

Key words: sex chromosome, fluroscence in situ hybridization, preimplantation genetic diagnosis