百草枯对金鱼血清过氧化物酶和酯酶表达的影响

doi:10.3969/j.issn.1004-616x.2010.05.015

Carcinogenesis, Teratogenesis & Mutagenesis ›› 2010, Vol. 22 ›› Issue (5): 391-393.doi: 10.3969/j.issn.1004-616x.2010.05.015

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Effect of paraquat on the expressions of peroxidase and esterase in goldfish serum

ZHANG Xiao-hong, ZHANG Hu-fang, WANG Qing

Department of Biology, Taiyuan Teachers College, Taiyuan 030031,Shanxi, China

Received:2010-04-06 Revised:2010-06-07 Online:2010-09-30 Published:2010-09-30
Contact: ZHANG Hu-fang

Abstract

Abstract: OBJECTIVE: To explore the effect of herbicide paraquat on the expression of peroxidase(POD) and esterase(EST) in Carassius auratus serum. METHODS: The 0.5,1.0,1.5 and 2.0 ml/L concentrations of paraquat were prepared according its 24 h LC50， Carassius auratus were treated with 0.1 ml/g dose of each concentration. SDS_polyarylamide gel were used to test the expressions of POD and EST. RESULTS: Compared with the control group, the four treated groups showed significant variety. The activities of POD and EST increased or decreased at low concentration，absent or increased at high paraquat concentration,concentration and activity had dose_effect relationship. CONCLUSION: The toxicity of paraquat on POD and EST varied in Carassius auratus.

Key words: paraquat, Carassius auratus, serum, peroxidase, esterase

ZHANG Xiao-hong, ZHANG Hu-fang, WANG Qing. Effect of paraquat on the expressions of peroxidase and esterase in goldfish serum[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2010, 22(5): 391-393.

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Effect of paraquat on the expressions of peroxidase and esterase in goldfish serum

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