nm23-H1基因稳定转染肺癌细胞的鉴定

doi:10.3969/j.issn.1004-616x.2011.02.015

›› 2011, Vol. 23 ›› Issue (2): 145-147.doi: 10.3969/j.issn.1004-616x.2011.02.015

• 检测研究 • Previous Articles Next Articles

Validation of nm23-H1 gene transfection in lung cancer cells

ZHENG Hai-xia1,ZHOU Qin-hua2，SHEN Dong-lan3,PENG An3，HE Yan-lin3，CAO Ju-xiu1

1. Department of Health Examination, Peking University Shenzhen Hospital, Shenzhen 518000; 2. General Hospital of Tianjin Medical University , Tianjin 300052; 3. Department of Oncology, Peking University Shenzhen Hospital, Shenzhen 518000, Guangdong, China

Received:2010-10-29 Revised:2010-11-01 Online:2011-03-30 Published:2011-03-30

Abstract

Abstract: nm23-H1 gene was transfected into nm23-H1 deletion human large lung cancer cell L9981 by stable，continuous and high effective gene transfection method. METHODS: nm23-H1 gene expression carrier was established, nm23-H1 was transfected in L9981 cell line and the expression of nm23-H1 was confirmed by gene transduction and protein translation. RESULTS: Transfected cell line showed nm23-H1 gene and protein expressions. CONCLUSION: The expression of nm23-H1 by L9981 cell line provides support for utilizing nm23-H1 as a potential therapy in lung cancer.

Key words: nm23-H1, gene trasfection, lung cancer, tumor metastasis

ZHENG Hai-xia1,ZHOU Qin-hua2，SHEN Dong-lan3,PENG An3，HE Yan-lin3，CAO Ju-xiu1. Validation of nm23-H1 gene transfection in lung cancer cells[J]. , 2011, 23(2): 145-147.

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Validation of nm23-H1 gene transfection in lung cancer cells

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