骨桥蛋白和原癌基因c-Met在胃癌中的表达及其临床意义

doi:10.3969/j.issn.1004-616x.2013.05.006

Carcinogenesis, Teratogenesis & Mutagenesis

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Expressions and significances of osteopontin and c-Met in gastric cancer

MA Gui-fang¹，CAI Hong-xing¹，DING Feng-yun¹，LIU Ran²，LI Yun-hui²

(1. Department of Inspection, Yancheng Institute of Health Science, Yancheng 224006; 2. Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing Jiangsu, China))

Received:2013-04-26 Revised:2013-06-17 Online:2013-09-30 Published:2013-09-30
Contact: MA Gui-fang,E-mail：ma_guif@126.com

Abstract

Abstract:

OBJECTIVE: To investigate the relationship between the expressions of osteopontin(OPN) and c-Met and its clinical significance in gastric cancer tissue. METHODS：Immunohistochemical method was used to detect the expression levels of OPN protein and c-Met protein in 69 tissue specimens from patients with gastric cancer，20 cases with nontypical hyperplasia，and 20 normal gastric mucosa specimens. We also investigated the relationships between their expressions and clinicopathological parameters；real-time quantitative reverse transcription-polymerase chain reaction(real-time PCR) was performed in 15 fresh gastric cancer samples and the corresponding normal tissue to detect mRNA expressions of OPN gene and c-Met gene. RESULTS：The positive rate of OPN was 69.6% in gastric cancer，which was significantly higher than 40.0% in nontypical hyperplasia tissues and 30.0% in normal gastric mucosa (P<0.05). The expression of OPN protein was closely correlated to the depth of invasion，TNM stage and lymph node metastasis. The expressions of OPN gene in gastric cancer tissues and normal gastric mucosa specimens were 2.08 and 0.46 times，respctively. The expression of OPN gene in gastric cancer tissues was significantly higher than normal gastric mucosa specimens (P<0.01). The positive rate of c-Met was 65.2% in gastric cancer，which was significantly higher than 30.0% in nontypical hyperplasia tissues and 20.0% in normal gastric mucosa (P<0.01). The expression of c-Met protein was closely correlated to the lymph node metastasis and TNM stage. The expressions of c-Met gene in gastric cancer tissues and normal gastric mucosa specimens were 0.21 and 0.03 times，respectively. The expression of c-Met gene in gastric cancer tissues was significantly higher than normal gastric mucosa specimens (P<0.01) . CONCLUSION：Increased expressions of OPN and c-Met may play important roles in the pathogenesis and progression of gastric cancer.

Key words: gastric cancer, metastasis, osteopontin, c-Met

MA Gui-fang，CAI Hong-xing，DING Feng-yun，LIU Ran，LI Yun-hui. Expressions and significances of osteopontin and c-Met in gastric cancer[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2013.05.006.

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Expressions and significances of osteopontin and c-Met in gastric cancer

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