长期暴露的邻苯二甲酸二(2-乙基己基)酯在雌性大鼠体内的代谢及对肝脏的影响

doi:10.3969/j.issn.1004-616x.2017.03.010

Abstract

Abstract: OBJECTIVE:To study the effect of di(2-ethylhexyl) phthalate (DEHP) on metabolism in livers of female rats. METHODS:64 female Wistar rats were randomly divided into four groups:control (corn oil) and 150,300,600 mg/mL DEHP groups with 16 rats per group. Different doses of DEHP were administered via gavage,daily in volume of 2.5 μL/g,for 6 months. At 3 and 6 months,rat urine samples were collected. Rats were sacrificed at 6 months later. The liver,spleen and kidney were removed,and the ratio of viscera was calculated. Liver tissue sections were prepared and their morphological changes were observed by HE staining. The contents of DEHP and its metabolite,mono-ethylhexyl phthalate (MEHP),in urine were measured by high performance liquid chromatography (HPLC) at 2 time points. The content of MEHP in DEHP and its metabolite,mono-ethylhexyl phthalate,was measured by high performance liquid chromatography (HPLC). RESULTS:Compared with the control group,there was no significant difference in the general growth of the rats in each dose group,but the ratio of the dirty body in the liver increased. HE staining showed slight changes in the liver tissues of the 300 and 600 mg/mL DEHP rats. At 3 months,the levels of DEHP and MEHP in the urine of the 300 and 600 mg/mL DEHP groups were significantly increased with the increase of DEHP dose,the difference was statistically significant (P < 0.05). At 6 months,the levels of DEHP and MEHP in the urine of the 600 mg/mL DEHP group were higher than those of the other three groups (P < 0.05). CONCLUSION:DEHP in DEHP and DEHP and MEHP in the female rats were increased which exceeded the normal metabolic capacity,therefore,the increase might have caused some damage to the liver.

Key words: di(2-ethylhexyl)phthalate, mono-ethylhexyl phthalate, high performance liquid chromatography, metabolism, liver damage

CLC Number:

R944.6

DONG Jin, DONG Xinwen, ZHAO Yue, GUO Jipeng, WANG Zhanju, NA Xiaolin. Prolonged exposure to di(2-ethylhexyl) phthalate on metabolism in female rats[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(3): 211-215.

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Prolonged exposure to di(2-ethylhexyl) phthalate on metabolism in female rats

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