解旋酶DDX46基因对食管鳞癌Eca-109细胞增殖及凋亡的影响

doi:10.3969/j.issn.1004-616x.2017.03.011

Abstract

Abstract: OBJECTIVE:To explore the effect of DDX46 knockdown by RNA interference on proliferation and apoptosis in esophageal squamous cell carcinoma Eca-109 cells. METHODS:DDX46 mRNA expression in Eca-109 and normal esophageal epithelium cell Het-1A were quantified using quantitative real-time PCR (qPCR). Then,DDX46 -shRNA-mediated RNA interference was applied to silence DDX46 in Eca-109. Subsequently,cell viability was measured by MTT assay,cell colony-forming capacity was measured by colony formation assay,cell cycle progression and apoptosis were determined by flow cytometry. Western blot analysis was used to detect changes of apoptosis signaling molecules. RESULTS:DDX46 mRNA was significantly up-regulated in Eca-109 cells compared with that in Het-1A cells (P < 0.01). DDX46 knockdown in Eca-109 led to reduced cell proliferation (P < 0.01),reduced number and size of cell colonies (P < 0.01),increased percentage of G1-phase cells,decreased percentage of S-phase cells (P < 0.05),and increased percentage of apoptotic cells (P < 0.01). Western blot analysis showed that the knockdown effectively suppressed the expression of DDX46 and up-regulated the expression of cleaved Caspase-3 and cleaved PARP-1 (P < 0.01). CONCLUSION:DDX46 mRNA was over-expressed in Eca-109 cells. Targeted silencing of the DDX46 gene inhibited cell proliferation,arrested cell cycle in the G0/G1 phase,and induced cell apoptosis. The DDX46 silencing effect could be mediated by positive regulation of the apoptosis signaling pathway which played the roles of inhibiting Eca-109 cells growth and inducing apoptosis.

Key words: esophageal squamous cell carcinoma, RNA helicase, DDX46, proliferation, apoptosis

CLC Number:

R730.2

GAO Hua, SONG Tieniu, LI Bin, YANG Jianbao, ZHU Duojie, GUO Quanwei. Effect of RNA helicase gene DDX46 knockdown on proliferation and apoptosis in esophageal squamous cell carcinoma Eca-109 cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(3): 216-221,229.

References

[1] ZHENG R S,ZENG H M,ZHANG S W,et al. National estimates of cancer prevalence in China,2011[J]. Cancer Lett,2016,370(1):33-38.
[2] SONG Y M,LI L,OU Y W,et al. Identification of genomic alterations in oesophageal squamous cell cancer[J]. Nature, 2014,509(7498):91-95.
[3] LINDER P,FULLER-PACE F V. Looking back on the birth of DEAD-box RNA helicases[J]. Biochim Biophys Acta,2013, 1829(8):750-755.
[4] OWTTRIM G W. RNA helicases:diverse roles in prokaryotic response to abiotic stress[J]. RNA Biol,2013,10(1):96-110.
[5] HOOPER C,HILLIKER A. Packing them up and dusting them off:RNA helicases and mRNA storage[J]. Biochim Biophys Acta,2013,1829(8):824-834.
[6] ROBERT F,PELLETIER J. Perturbations of RNA helicases in cancer[J]. Wiley Interdiscip Rev RNA,2013,4(4):333-349.
[7] LI M,MA Y C,HUANG P,et al. Lentiviral DDX46 knockdown inhibits growth and induces apoptosis in human colorectal cancer cells[J]. Gene,2015,560(2):237-244.
[8] ADMONI-ELISHA L,NAKDIMON I,SHTEINFER A,et al. Novel biomarker proteins in chronic lymphocytic leukemia:impact on diagnosis,prognosis and treatment[J]. PLoS One, 2016,11(4):e0148500.
[9] LI B,LI Y M,HE W T,et al. Knockdown of DDX46 inhibits proliferation and induces apoptosis in esophageal squamous cell carcinoma cells[J]. Oncol Rep,2016,36(1):223-230.
[10] ZIELSKE S P,STEVENSON M. Importin 7 may be dispensable for human immunodeficiency virus type 1 and simian immunodeficiency virus infection of primary macrophages[J]. J Virol,2005,79(17):11541-11546.
[11] LINDER P,LASKO P F,ASHBURNER M,et al. Birth of the D-E-A-D box[J]. Nature,1989,337(6203):121-122.
[12] KOSOWSKI T R,KEYS H R,QUAN T K,et al. DExD/H-box Prp5 protein is in the spliceosome during most of the splicing cycle[J]. RNA,2009,15(7):1345-1362.
[13] LIANG W W,CHENG S C. A novel mechanism for Prp5 function in prespliceosome formation and proofreading the branch site sequence[J]. Genes Dev,2015,29(1):81-93.
[14] HOZUMI S,HIRABAYASHI R,YOSHIZAWA A,et al. DEAD-box protein DDX46 is required for the development of the digestive organs and brain in zebrafish[J]. PLoS One, 2012,7(3):e33675.
[15] ABDELHALEEM M,MALTAIS L,WAIN H. The human DDX and DHX gene families of putative RNA helicases[J]. Genomics,2003,81(6):618-622.
[16] PORTER A G,JÄNICKE R U. Emerging roles of caspase-3 in apoptosis[J]. Cell Death Differ,1999,6(2):99-104.
[17] 陈海军,刘玉琴,李雨泽,等. 维生素E琥珀酸酯对人食管癌TE-1和Eca-109细胞增殖及凋亡的影响[J]. 癌变·畸变·突变,2015,27(5):357-360.
[18] LEE A S,KRANZUSCH P J,DOUDNA J A,et al. eIF3d is an mRNA cap-binding protein that is required for specialized translation initiation[J]. Nature,2016,536(7614):96-99.

Effect of RNA helicase gene DDX46 knockdown on proliferation and apoptosis in esophageal squamous cell carcinoma Eca-109 cells

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