丁草胺对金鱼主要器官酯酶和过氧化物酶同工酶的影响

doi:10.3969/j.issn.1004-616x.2018.04.008

Abstract

Abstract: OBJECTIVE: To explore the environmental risk of butachlor on aquatic organisms. METHODS: 0.4,0.8 and 1.2 mL/L concentrations of butachlor were prepared according to 24 h-LC₅₀. Carassius auratus were administered by intraperitoneal injection with 0.1 mL/g dose of each concentration for 24 h. PAGE method was used to detect the activity of EST and POD isozyme. RESULTS: EST and POD isozyme in the hearts of C. auratus were not expressed in the control group but was more than 50 times (in 1.2 mL/L) that of the control group. With increased concentrations of butachlor,the activity of EST isozyme in hepatopancreas gradient attenuated while the POD decreased followed by increased. The activities of EST isoenzymes in gill and kidney were 2 times and 3 times higher than those in the control group at 0.4 mL/L,respectively. In addition,the activities of EST and POD isoenzymes in gill and kidney disappeared at 0.8 mL/L and re-expressed at 1.2 mL/L. CONCLUSION: The concentration of butachlor and the activity of EST and POD isozyme in C. auratus show dose-effect relationship. Butachlor with medium concentration (0.8 mL/L) was at risk for EST and POD isozyme in gill and kidney of C. auratus.

Key words: butachlor, Carassius auratus, esterase, peroxidase

CLC Number:

X503.2

ZHANG Xiaohong, GAO Jinhua, FAN Xuankun, CHEN Wanshan, LI Ruimei. Effect of butachlor on EST and POD isoenzyme in Carassius auratus[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(4): 286-290,296.

References

[1] 卢静静,卢振兰,张瑾,等. 除草剂丁草胺的研究进展[J]. 绿色科技,2015,(2):195-196.
[2] 卢静静. 稻田水中除草剂丁草胺对鲫鱼毒性的研究[D]. 长春:吉林农业大学,2015.
[3] CAPEL P D, MA L, SCHROYER B R, et al. Analysis and detection of the new corn herbicide acetochlor in river water and rain[J]. Environm Sci Tech,1995,29(6):1702-1705.
[4] ATEEQ B A, FARAH M, AHMAD W. Detection of DNA damage by alkaline single cell gel electrophoresis in 2,4-dichlorophenoxyacetic-acidand butachlor-exposed erythrocytes of Clarias batrachus[J]. Ecotox Environ Safe,2005,62(3):348-354.
[5] 韦龙旭. 丁草胺、噻虫嗪、嘧菌酯对青萍的毒性影响研究[D]. 长春:吉林农业大学,2016.
[6] 徐华东. 基于核磁共振代谢组学的除草剂丁草胺对金鱼的毒性研究[D]. 南京:南京理工大学,2015.
[7] SINGN YADAV A, BHATNAGAR A, KAUR M. Aberrations in the chromosomes of cirrhinus mrigala(Hamilton) upon exposure to Butachlor[J]. J Toxicol,2013,7(21):858-865.
[8] 刘迎,胡燕,姜蕾,等. 5种酰胺类除草剂对斑马鱼胚胎发育的毒性效应[J]. 农药,2014,53(11):806-808.
[9] CHANG J H,LIU SY,ZHOU S L,et al. Effects of butachlor on reproduction and hormone levels in adult Zebrafish (Danio rerio)[J]. Exp Toxicol Pathol,2013,65(1/2):205-209.
[10] 姜建国,熊全沫,姚汝华. 青鱼不同组织中同工酶的表达模式[J]. 水生生物学报,1997,21(4):353-358.
[11] 罗曼,蒋立科,刘颖,等. 野生与养殖长吻鳜血液及不同器官同工酶的比较[J]. 应用与环境生物学报,2000,19(5):447-451.
[12] 张晓红. 丁草胺对金鱼血清同工酶表达的影响[J]. 山西师范大学学报(自然科学版),2010,24(1):81-84.
[13] 张晓红. 除草剂对金鱼血清酯酶同工酶的影响[J]. 安徽农业科学,2010,38(8):4121-4122,4132.
[14] XIANG Q,XU B,DING Y. Oxidative stress response induced by butachlor in zebrafish embryo/larvae:the protective effect of vitamin c[J]. Bull Environ Contam Toxicol,2018,100(2):208-215.
[15] 张舒婕,李广科,桑楠. NO₂吸入诱导小鼠肺组织上皮细胞间质转化及单酰基甘油酯酶抑制的保护作用[J]. 环境科学学报,2016,36(11):4272-4277.
[16] 常菊花. 丁草胺对斑马鱼的内分泌干扰效应研究[D]. 南京:南京农业大学,2012.
[17] 涂青松,何剪太,陈伟. 抑制过氧化物酶1表达对肝癌细胞放射敏感性的影响[J]. 中国普通外科杂志,2016,25(2):245-251.
[18] 涂青松,何剪太. 过氧化物酶1对人肝癌细胞HepG2细胞皮下移植瘤生长的影响[J]. 中国现代医学杂志,2016,26(8):11-16.

Effect of butachlor on EST and POD isoenzyme in Carassius auratus

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 12

Recommended Articles

Metrics

Comments

[1]	LIU Xiaoxia, LI Weixia, LI Zhijia, SU Zhenjun, DUAN Xiaohui, HAN Weiwen, WANG Yunxiao, HE Xiaolei, Lu Linlin, JIA Jinhai. Correlations between glutathione peroxidase in serum and clinicopathological outcomes of advanced non-small cell lung cancer [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(4): 286-290,295.
[2]	XU Haiming, HAO Yanxing, CHEN Yanan, YANG Huifang, LI Jiangping, ZHU Lingqin, REN Binbin, DE Xiaoming. Optimization for determination of cholinesterase in human serum using the hydroxylamine ferric chloride method [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(2): 145-148,154.
[3]	SHAO Shuai, WEI Zhiquan, ZHANG Wei, TONG Peng, WANG Chunyan, QI Xuesong, GOU Qiao. Effects of glutathione peroxidase 1 overexpression on DNA oxidative damage and phenotype of malignant BERP35T1 cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2016, 28(1): 8-13,18.
[4]	JIN Lei, WANG Shuai, WANG Xin, HAI Chunxu, LI Wenli. Induction of insulin resistance by palmitic acid in BRL-3A cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2016, 28(1): 46-50,55.
[5]	BO Cunxiang, ZHANG Zhenling, SAI Linlin, ZHANG Fang, YANG Zhifeng, XIE Lin, JIA Qiang. Effects of 2,4-dichlorophenoxyacetic acid on offsprings' development and oxidative stress [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2015, 27(3): 221-224.
[6]	ZHAO Minxian, YAO Xinya, CAO Zhengying, WANG Cannan. Toxicokinetics and toxicodynamics of chlorpyrifos in rats following one single subcutaneous exposure [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2015, 27(2): 129-133,145.
[7]	WU Hui，YI Nan-nan，LI Ting-ting，HE Xian-song，ZHAO Min-xian，YAO Xin-ya，WANG Can-nan. Combined toxic effect of chorpyriphos and dimethoate on cholinesterase in rats [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2013, 25(1): 31-34.
[8]	ZHANG Xiao-hong, ZHANG Hu-fang, WANG Qing. Effect of paraquat on the expressions of peroxidase and esterase in goldfish serum [J]. , 2010, 22(5): 391-393.
[9]	DUAN Lian;CHENG Yi-bin;JIN Yin-long. Effects of Copper Intake and Copper-zinc Ratio on Rat Lipid Peroxidation in Copper Deficiency [J]. , 2009, 21(6): 447-451.
[10]	ZHU Xin1, LIAN Ling-jun1,WANG Yan1,LIU Hui-gang1,XU Li-hong1,2. Tributyltin Exposure Leads to Acetylcholinesterase Activity Elevation in Rat Brain [J]. , 2006, 18(4): 292-293.
[11]	WEI Chuan-bao , YU Mao-yun. STUDY ON THE MUT AGENICITY OF ARGININE ESTERASE FROM AGKISTRODON HALYS USSURIENSIS VENOM [J]. , 2003, 15(4): 237-239.
[12]	WEI Chuan-bao1, 2, CHEN Fei1. TERATOGENICITY TEST OF ARGININE ESTERASE FROM AGKISTRODON HALYS USSURIENSIS VENOM [J]. , 2003, 15(2): 97-100.