Carcinogenesis, Teratogenesis & Mutagenesis ›› 2020, Vol. 32 ›› Issue (4): 304-308.doi: 10.3969/j.issn.1004-616x.2020.04.010

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Risk analysis of HPV-52 infection and cervical squamous epithelial lesions in Shantou, China

WANG Yuanyuan1, CHEN Yichao2, ZHENG Jie1, PENG Yanyan3, WANG Shaohong1   

  1. 1. Department of Pathology, Shantou Central Hospital, Shantou 515041;
    2. Department of Clinical Research Center, Shantou Central Hospital, Shantou 515041;
    3. Department of Laboratory Medicine, Shantou Central Hospital, Shantou 515041, Guangdong, China
  • Received:2020-01-08 Revised:2020-05-19 Online:2020-07-31 Published:2020-08-01

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Abstract: OBJECTIVE: To explore pathogenic characteristics and risk of human papillomavirus-52 (HPV-52) infection in cervical squamous cell lesions in Shantou,China. METHODS: 37 700 cases of HPV typing and thin-layer liquid-based cytology (LCT),and 3 924 cases of cervical biopsy were collected in the Shantou Central Hospital from 2015 to 2018. HPV was detected by using the medical nucleic acid molecule rapid hybridization genotype kit which contained 15 high-risk HPV (HPV-16,-18,-31,-33,-35,-39, -45,-51,-52,-53,-56,-58,-59,-66,and -68) and 6 low-risk HPV (HPV-6,-11,-42,-43,-44, CP8304). Cytology examination was classified according to the TBS (the Bethesda system) classification standard (2014 version),and was divided into negative for intraepithelial lesion or malignancy (NILM),atypical squamous cells of undetermined significance (ASC-US), low-grade squamous intraepithelial lesion (LSIL), atypical squamous cells without exclusion of HSIL (ASC-H),high grade squamous intraepithelial lesion(HSIL) and squamous cell carcinoma (SCC). The database was established by SPSS 19.0. The rate comparison of the counted data was performed by using frequency distribution. The risk assessment was performed by using a chi-square test 2×2 list. RESULTS: The total infection rate of HPV-52 in 37 700 patients was 3.55% (1 338/37 700). There were 882 cases of HPV-52 single infection and 456 cases of HPV-52 multiple infection, including 313 cases of double infection and 143 cases of triple and above.The three most common genotype of HPV-52 multiple infection were HPV-58/52 (12.46%,39/313),HPV-53/52 (11.18%,35/313),HPV-16/52 (11.18%,35/313). The most common genotype was HPV-16/52 in ASC-US and above lesions,especially in HSIL. Risk analysis of cervical biopsy showed that HPV-52/16 increased the risk of HSIL/SCC,compared with HPV-52 single infection (P < 0.01,OR=8.27),and increased the risk from HSIL to SCC (P=0.01,OR=12.65). Multiple infections of HPV-52/16 increased the risk from HSIL to SCC,compared with multiple infections that included HPV-52 but not HPV-16 (P < 0.01,OR=3.36),and increased the risk from HSIL to SCC (P=0.03, OR=1.29). CONCLUSION: HPV-52 was the most common genotype in Shantou area but the patients suffered mainly HSIL lesions. On the other hand, infection with HPV-16 increased the risk of precancerous and cancerous lesions.

Key words: human papilloma virus, cervical cancer, multiple infection, genotype

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