Carcinogenesis, Teratogenesis & Mutagenesis ›› 2022, Vol. 34 ›› Issue (2): 144-148.doi: 10.3969/j.issn.1004-616x.2022.02.013

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Reproductive toxicity of Capparis spinosa fruits pain gel paste in SD rats

XU Wenli1, HE Jiang2, LI Shufang1, WANG Xingang1, ZHANG Yajuan1, LIU Huan1, YUE Juan1, LI Jianguo1, YANG Weijun2,   

  1. 1. Drug Safety Evaluation Center in China Institute for Radiation Protection, Taiyuan 030006, Shanxi;
    2. Institute of Materia Medica of Xinjiang, Urumqi 830004, Xinjiang, China
  • Received:2021-08-05 Revised:2021-11-22 Published:2022-04-07

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Abstract: OBJECTIVE：To evaluate reproductive toxicity Capparis spinosa fruits pain gel paste in SD rats. METHODS： SD rats were randomly divided into five groups： excipient control (excipient)， positive control (cyclophosphamide) and Capparis high-dose (117.8 g/m2)，medium-dose(58.9 g/m2) and low-dose (29.5 g/m2) groups. There were 25 males and 25 females in each group. The rats were given by transdermal delivery once a day. The male rats received administration for 4 weeks before mating. Female rats received administration of 2 weeks before mating to the 6th days after pregnancy. Positive controls received single intraperitoneal injection (0.1 g/kg cyclophosphamide) on D1 of administration in male and female rats. The rats were observed of the general condition， weight， food intake and mating during the experiment. When the female rats were confirmed to be pregnant，the male rats were executed and their epididymis，testicle and spermatozoa were examined. The female rats were executed on the 14th day of pregnancy， and the uterus， ovaries and embryos were studied. RESULTS：There was no significant effect on weight and feed consumption of rats and pregnant rats. There were no significant effects on the mating rates，transverse diameters of testes， sperm counts of epididymis，mobility and malformation rates，epididymis and testicular organ weights. There were no significant effects on pregnancy rates， uterine fetal weights， mean corpus luteum numbers， mean implantation numbers， mean live fetus numbers， mean stillbirth numbers， mean absorbed fetus numbers， stillbirth rates and ovarian organ weights. In the high-dose group， the pre-implantation loss rates were increased (P<0.05) and the implantation rates were decreased (P<0.05). In the low- dose group， the pre-implantation loss rates were decreased (P<0.01) and the implantation rates were increased (P<0.01). These had a certain dose- effect relationship. CONCLUSION： Capparis spinosa fruits pain gel had no significant toxic effect on the fertility of male rats， while the high dose (117.8 g/m2) showed some reproductive toxicity in female rats. The medium (58.9 g/m2) and low (29.5 g/m2) doses showed no significant toxicity on female fertility.

Key words: Capparis spinosa fruits pain gel, SD rats, general reproductive toxicity, drug safety evaluation

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