人参培养须根的大鼠致畸试验

doi:10.3969/j.issn.1004-616x.2022.06.010

Abstract

Abstract: OBJECTIVE:To evaluate teratogenicity of cultured ginseng fibrous roots in rats. METHODS:80 female and 40 male SD rats were caged in a ratio of 2:1 for mating. Pregnant rats were randomly divided into high-,medium-,and low-dose groups (2,4,8 g/kg,respectively,equivalent to the recommended amount of 60.5,121,242 times according to body mass) and control group,with 16 in each group. The rats were continuously given ginseng between 6-15 days of conception,and were executed on the 20th day. Caesarean sections were conducted to remove the uterus to inspect the quality,and to check the number of implantations,absorption of fetuses,early stillbirth,late stillbirth,and the numbers and abnormalities of live fetuses. Some fetal pups were examined for appearance,and bone and visceral abnormalities. RESULTS:Compared with the control group,there were no significant differences in the body mass,uterine quality,average number of live births,and length and body quality of pregnant rats. The appearance,organs and bones of the fetal rats were examined. Each dose group,there was no obvious abnormalities and deformities in the negative control group. CONCLUSION:The cultured fibrous roots of ginseng showed no teratogenic effects under the conditions of our investigation.

Key words: ginseng, cultured ginseng fibrous root, teratogenicity, safety evaluation

CLC Number:

R394.6

LIU Huan, LIANG Fei, LI Ying, WU Shasha, CAO Can, XU Jianwen, LIU Ying. Study on teratogenicity of cultured ginseng fibrous root in rats[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2022, 34(6): 459-462.

References

[1] 宋齐. 人参主要化学成分及皂苷提取方法研究进展[J]. 人参研究, 2019, 31(4):43-46.
[2] 于雪妮, 冯小刚, 张建民, 等. 人参化学成分与药理作用研究新进展[J]. 人参研究, 2019, 31(1):47-51.
[3] WANG Y, LIU Y, ZHANG X Y, et al. Ginsenoside Rg1 regulates innate immune responses in macrophages through differentially modulating the NF-KB and PI3K/Akt/mTOR pathways[J]. Int Immunopharmacol, 2014, 23(1):77-84.
[4] IM D S. Pro-resolving effect of ginsenosides as an anti-inflammatory mechanism of Panax ginseng[J]. Biomolecules, 2020, 10(3):444.
[5] SHALABY A S G, RAGAB T I M, MEHANY A B M, et al. Antitumor and prebiotic activities of novel sulfated acidic polysaccharide from ginseng[J]. Biocatal Agric Biotechnol, 2018, 14:402-409.
[6] 翟兵中, 曲雪峰, 胡文力, 等. 人参不定根生产、生物活性成分及应用现状[J]. 食品安全质量检测学报, 2021, 12(15):6097-6104.
[7] MURTHY H N, GEORGIEV M I, KIM Y S, et al. Ginsenosides:prospective for sustainable biotechnological production[J]. Appl Microbiol Biotechnol, 2014, 98(14):6243-6254.
[8] 刘娟, 纪瑞锋, 陈同, 等. 人参皂苷生物合成基因组织表达特性的研究[J]. 中国中药杂志, 2017, 42(13):2453-2459.
[9] 郝悦君, 孙浩丁, 车成来, 等. 2种培养方式下人参不定根生长动态观察及活性物质含量测定[J]. 延边大学农学学报, 2020, 42(2):14-20.
[10] 蔡铁全, 吴昊, 李莹, 等. 人参培养须根的毒理安全性初步研究[J]. 毒理学杂志, 2021, 35(3):262-266.
[11] 吕静薇, 姜宁, 王海霞, 等. 基于功能性防护的人参保健食品研究进展[J]. 人参研究, 2020, 32(4):45-50.
[12] 蒋常鹏, 李昕曈, 曹文正, 等. 基于人参功能活性的保健食品开发现状与展望[J]. 保鲜与加工, 2021, 21(11):113-120.
[13] BAQUE M A, MOH S H, LEE E J, et al. Production of biomass and useful compounds from adventitious roots of high-value added medicinal plants using bioreactor[J]. Biotechnol Adv, 2012, 30(6):1255-1267.
[14] OCHOA-VILLARREAL M, HOWAT S, HONG S M, et al. Plant cell culture strategies for the production of natural products[J]. BMB Rep, 2016, 49(3):149-158.
[15] LIU P, YIN H J, XU Y J, et al. Effects of ginsenoside Rg1 on postimplantation rat and mouse embryos cultured in vitro[J]. Toxicol Vitro, 2006, 20(2):234-238.
[16] CHAN L Y, CHIU P Y, LAU T K. An in-vitro study of ginsenoside Rb1-induced teratogenicity using a whole rat embryo culture model[J]. Hum Reprod, 2003, 18(10):2166-2168.
[17] SEELY D, DUGOUA J J, PERRI D, et al. Safety and efficacy of Panax ginseng during pregnancy and lactation[J]. Can J Clin Pharmacol, 2008, 15(1):e87-e94.
[18] 中华人民共和国国家卫生和计划生育委员会. 食品安全国家标准致畸试验:GB 15193.14-2015[S]. 北京:中国标准出版社, 2015.

Study on teratogenicity of cultured ginseng fibrous root in rats

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