ICG-001对食管鳞癌细胞增殖的抑制作用及其分子机制

doi:10.3969/j.issn.1004-616x.2023.03.005

Abstract

Abstract: OBJECTIVE：To investigate effects of small molecule inhibitor ICG-001 on malignant phenotypes of esophageal squamous carcinoma (ESCC) cells and to explore its molecular mechanisms. METHODS：The ESCC cell lines KYSE410 and KYSE450 were treated with ICG-001 of 50 and 100 μmol/L，or with solvent DMSO as control. Treated cells were evaluated for cell proliferation viability，colony formation ability，cell cycle distribution and apoptosis. mRNA and protein expression of related molecules (SKP2，Survivin，and TCF4) were detected by real-time PCR (qPCR) and Western blot. RESULTS：Compared with the control group，ICG-001 treatment significantly inhibited the proliferation and colony formation ability of KYSE410 and KYSE450，and significantly induced G0/G1 phase arrest (P<0.01). In addition，expression levels of SKP2、Survivin and TCF4 were significantly decreased (P<0.01). CONCLUSION：ICG-001 significantly inhibited the proliferation viability and colony formation ability，and induced G0/G1 phase arrest of the two ESCC cell lines. The mechanisms may be related to the inhibition of b-catenin/TCF4 transcriptional activity and the expression of downstream molecules：Survivin and SKP2.

Key words: ICG-001, esophageal squamous cell carcinoma, proliferation, cell cycle, small molecule inhibitor

CLC Number:

R735.1

SHI Jianhong, CHEN Dingxiong, LU Xiaotong, HAO Jiajie, CAI Yan, WANG Mingrong, ZHANG Yu. Inhibitory effect of ICG-001 on proliferation of esophageal squamous carcinoma cells and its molecular mechanisms[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2023, 35(3): 187-191,196.

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Inhibitory effect of ICG-001 on proliferation of esophageal squamous carcinoma cells and its molecular mechanisms

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