miR-495通过抑制RNF113A的表达调控食管鳞癌细胞恶性表型

doi:10.3969/j.issn.1004-616x.2023.03.008

Abstract

Abstract: OBJECTIVE：To explore effects of microRNA-495 (miR-495) on malignant phenotype of esophageal squamous carcinoma cells and its molecular mechanisms. METHODS：miR-495 mimics and miR-495 inhibitors，and the corresponding random sequence control were transfected into esophageal cancer cells Eca109. Real-time fluorescent quantitative polymerase chain reaction PCR (qPCR) was used to detect expression of miR-495，CCK-8 to detect cell proliferation，flow cytometry to detect cell cycle and apoptosis，transwell to detect cell migration and invasion ability，qPCR and western blot to detect expression of mRNAs and proteins of downstream target gene RNF113A. RESULTS：Compared with the control group，the miR-495 mRNA levels in the miR-495 mimics transfected group were significantly increased (P<0.05)，and in the miR-495 inhibitor transfected group was significantly decreased (P<0.05). There was no significant changes in cell proliferation among the groups (P>0.05). The migration and invasion numbers in the miR-495 mimics transfected group were significantly decreased (P<0.01)，and the apoptosis rate was significantly increased (P<0.01). The levels of cells blocked in the G0/G1 phase (P<0.01) were significantly more than that in the controls. The migration and invasion numbers in the miR-495 inhibitor transfected group were significantly increased (P<0.01)，and the apoptosis rate was significantly decreased (P<0.01). The proportion of S phase cells in the miR-495 inhibitor transfected group was increased (P<0.05)，and the difference was statistically significant. Compared with the control group，expression of the RNF113A protein in the miR-495 mimics transfected group was down-regulated (P<0.05)，and expression of the RNF113A in the miR-495 inhibitor transfected group was up-regulated (P<0.05). However，the mRNA levels of RNF113A did not change significantly (P>0.05). CONCLUSION：miR-495 promoted cell apoptosis，inhibited cell migration and invasion ability，and regulated cell cycle distribution. Thus，miR-495 may mediate malignant phenotype of esophageal squamous carcinoma cells by regulating expression of the RNF113A protein.

Key words: esophageal cancer, miR-495, RNF113A, malignant phenotype

CLC Number:

R735.1

FU Jing, LIU Qing, YU Haiye, WANG Lei. Regulation of malignant phenotype of esophageal squamous carcinoma cells by miR-495 via suppressing the expression of RNF113A[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2023, 35(3): 202-208,214.

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Regulation of malignant phenotype of esophageal squamous carcinoma cells by miR-495 via suppressing the expression of RNF113A

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