自噬在α粒子辐射诱发人支气管上皮细胞恶性转化中的作用

doi:10.3969/j.issn.1004-616x.2024.01.002

Abstract

Abstract: OBJECTIVE: To investigate the role of autophagy in malignant transformation of immortalized human bronchial epithelial cells BEP2D induced by alpha particle radiation. METHODS: Western blot was used to detect the expression of autophagy related proteins(LC3B-II, LC3B-I and P62) in alpha particles exposed BEP2D and in exposed malignant transformed cell line BERP35T-1. Intracellular autophagosomes were observed by transmission electron microscopy. BERP35T-1 cells were also treated with 40 and 60 μmol/L autophagy inhibitor chloroquine(CQ) and 25 pmol/L autophagy activator Rapamycin(Rapa), respectively, for detection of of LC3Bs and P62. Survival rates of cells were measured by CCK-8, invasion ability by the Transwell invasion assay, and the migration ability by the scratch healing assay. RESULTS: Compared with BEP2D cells, the numbers of autophagosomes in RH24 and BERP35T-1 cells were increased, LC3B-II/I protein ratio increased, and P62 protein expression decreased. After treatment with 40 and 60 μmol/L CQ for 48 h, the LC3B-II/I protein ratio and the expression level of P62 protein in BERP35T-1 cells were increased, and the cell survival rate, invasion number and scratch closure rate decreased(all P<0.01). After treatment with 25 pmol/L Rapa for48 h, the LC3B-II/I protein ratios were increased, the expression of P62 protein decreased, and the survival rate of BERP35T-1 cells, invasion number and scratch closure rate were increased(P<0.05 or 0.01).CONCLUSION: During the alpha particle-induced malignant transformation of BEP2D cells, autophagy was enhanced which was associated with increased ability of cell proliferation, invasion and migration.

Key words: autophagy, alpha particle, human bronchial epithelial cells, canceration, lung cancer

CLC Number:

R734.1

YANG Li, SHAO Shuai, WU Yunyun, WANG Chengfang, QU Gonglin, YAN Haoyu, GOU Qiao. Role of autophagy in alpha particle-induced malignant transformation of human bronchial epithelial cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2024, 36(1): 9-15,20.

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Role of autophagy in alpha particle-induced malignant transformation of human bronchial epithelial cells

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