水培生菜中锶的迁移富集及其生理影响

doi:10.3969/j.issn.1004-616x.2024.04.011

Abstract

Abstract: OBJECTIVE：The aim of this study was to investigate the migration and accumulation of strontium (Sr) in lettuce，as well as Sr's role on physiological effects in lettuce. METHODS：Lettuces were cultured using hydroponic method，with one control group (without Sr added) and five Sr treatment groups (0.1，0.5，1，5，and 10 mmol/L). The contents of calcium，iron，potassium，magnesium，sodium，phosphorus，sulfur and strontium in lettuce stem leaf and root tissues were detected by Inductively coupled plasma atomic emission spectrometry (ICP-AES)，and the distributions of Sr in lettuce stem leaf and root tissues cells were analyzed by scanning electron microscope coupled X-ray energy dispersive spectrometer (SEM-EDX). RESULTS：The mass fractions of Ca，S，Sr in lettuce stems and leaves，and Sr in roots showed statistically significant differences under different Sr treatment groups (P<0.05). The transportation factors (TF) of S treated with 5 and 10 mmol/L Sr was lower than that of control group (P<0.05)，and the TF values of Sr decreased with the increase of Sr concentration in culture solution. As the concentration of Sr in the hydroponic solution increased，the TF value of Sr showed a decreasing trend. The concentrations of S in the culture solution under 5 and 10 mmol/L Sr were significantly decreased compared with the control group，and the difference was statistically significant (P<0.05). Compared with the control group，the chlorophyll content (SPAD) values of lettuce treated with 5 and 10 mmol/L Sr were significantly increased (P<0.05). The weight percentage of Sr in lettuce tissue was as follows：leaf surface guard cell > epidermis，leaf cross section mesophyll ≈ epidermis，root cross section epidermis > pericycle > phloem. CONCLUSION：The mass fraction of Sr in lettuce root was higher than that in stem and leaf. With the increase of Sr treatment concentration，the proportions of Sr migrating from roots to leaves decreased，and the mass percentages of S in roots increased，and the SPAD values of leaves increased. The content of Sr absorbed by lettuce roots were mainly fixed on the epidermis of lettuce roots.

Key words: lettuce, strontium, migration, nutrient elements, SEM-EDX

CLC Number:

YAN Dong, LI Bin, LIANG Chenqing, HE Yingxue, FAN Li, JIANG Xiaoyan. Migration, accumulation and the physiological effects of strontium in hydroponic lettuce[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2024, 36(4): 309-314,319.

References

[1] SOUDEK P, VALENOVÁ S, VAVRÍKOVÁ Z, et al. ¹³⁷Cs and ⁹⁰Sr uptake by sunflower cultivated under hydroponic conditions [J]. J Environ Radioact, 2006, 88(3): 236-250.
[2] BURGER A, LICHTSCHEIDL I. Strontium in the environment: review about reactions of plants towards stable and radioactive strontium isotopes[J]. Sci Total Environ, 2019, 653: 1458-1512.
[3] DING K K, LIU S J, HE Y X, et al. Simulating the transfer of strontium-90 from soil to leafy vegetables by using strontium- 88[J]. Water Air Soil Pollut, 2016, 227(11): 414.
[4] SINGH S, EAPEN S, THORAT V, et al. Phytoremediation of ¹³⁷cesium and ⁹⁰strontium from solutions and low-level nuclear waste by Vetiveria zizanoides[J]. Ecotoxicol Environ Saf, 2008, 69(2): 306-311.
[5] SOWA I, WÓJCIAK-KOSIOR M, STRZEMSKI M, et al. Biofortification of soy (Glycine max L. Merr.) with strontium ions [J]. J Agric Food Chem, 2014, 62(23): 5248-5252.
[6] QI L, QIN X L, LI F M, et al. Uptake and distribution of stable strontium in 26 cultivars of three crop species: oats, wheat, and barley for their potential use in phytoremediation[J]. Int J Phytoremediation, 2015, 17: 264-271.
[7] GUPTA D K, SCHULZ W, STEINHAUSER G, et al. Radiostrontium transport in plants and phytoremediation[J]. Environ Sci Pollut Res Int, 2018, 25(30): 29996-30008.
[8] KOVÁČIK J, DRESLER S, STRZEMSKI M, et al. Nitrogen modulates strontium uptake and toxicity in Hypericum perforatum plants[J]. J Hazard Mater, 2022, 425: 127894.
[9] WANG X, CHEN C, WANG J L. Phytoremediation of strontium contaminated soil by Sorghum bicolor (L.) Moench and soil microbial community-level physiological profiles (CLPPs) [J]. Environ Sci Pollut Res Int, 2017, 24(8): 7668-7678.
[10] LIU J, FAN X T, JIANG Y Y, et al. Strontium alleviated the growth inhibition and toxicity caused by cadmium in rice seedlings[J]. Sci Total Environ, 2023, 904: 166948.
[11] TSIALTAS J T, MATSI T, BARBAYIANNIS N, et al. Strontium absorption by two Trifolium species as influenced by soil characteristics and liming[J]. Water Air Soil Pollut, 2003, 144(1): 363-373.
[12] MOYEN C, ROBLIN G. Occurrence of interactions between individual Sr²⁺- and Ca²⁺-effects on maize root and shoot growth and Sr²⁺ , Ca²⁺ and Mg²⁺ contents, and membrane potential: consequences on predicting Sr²⁺-impact[J]. J Hazard Mater, 2013, 260: 770-779.
[13] 朱宇恩, 赵烨, 徐东昱, 等. 旱柳(Salix matsudana koidz)体内Cu迁移特征的水培模拟研究[J]. 环境科学学报, 2011, 31(12): 2740-2747.
[14] YAN D, WANG S F, DING K K, et al. Strontium uptake and effect in lettuce and radish cultivated under hydroponic conditions[J]. Bull Environ Contam Toxicol, 2019, 103(3): 453- 460.
[15] 姜晓燕, 刘淑娟, 闫冬, 等. 锶在土培辣椒不同器官中的富集与迁移规律[J]. 癌变·畸变·突变, 2020, 32(4): 309-311, 316.
[16] 刘淑娟, 闫冬, 何映雪, 等. 三种蔬菜对土壤中Sr元素富集和转移特性的研究[J]. 中华放射医学与防护杂志, 2015, 35(5): 376-379.
[17] YAN D, DING K K, HE Y X, et al. Effect of strontium on nutrient uptake, physiological parameters, and strontium localization in lettuce[J]. Environ Sci Pollut Res Int, 2022, 29(23): 34874-34886.
[18] 闫冬, 丁库克, 何映雪, 等. 3种植物在水培方式下对锶的富集和迁移实验研究[J]. 癌变·畸变·突变, 2019, 31(1): 64-68.
[19] YAN D, DING K K, HE Y X, et al. Strontium uptake and effects in hydroponically cultivated celery[J]. Water Air Soil Pollut, 2020, 231(6): 279.
[20] 李奕奉, 闫冬, 何映雪, 等. 在水培方式下锶对白菜和油菜某些生理指标的影响[J]. 癌变·畸变·突变, 2016, 28(3): 223- 226.
[21] ROBINSON N J, TOMMEY A M, KUSKE C, et al. Plant metallothioneins[J]. Biochem J, 1993, 295(Pt 1): 1-10.
[22] COBBETT C, GOLDSBROUGH P. Phytochelatins and metallothioneins: roles in heavy metal detoxification and homeostasis[J]. Annu Rev Plant Biol, 2002, 53: 159-182.
[23] YANG L P, ZENG J, WANG P, et al. Sodium hydrosulfide alleviates cadmium toxicity by changing cadmium chemical forms and increasing the activities of antioxidant enzymes in salix[J]. Environ Exp Bot, 2018, 156: 161-169.
[24] GUAN M Y, ZHANG H H, PAN W, et al. Sulfide alleviates cadmium toxicity in Arabidopsis plants by altering the chemical form and the subcellular distribution of cadmium[J]. Sci Total Environ, 2018, 627: 663-670.
[25] SAHIN O, BABAR S K, DENIZ K, et al. Strontium biofortification in soil and hydroponic grown tomato and lettuce [J]. J Plant Nutr, 2024, 47(9): 1456-1463.

Migration, accumulation and the physiological effects of strontium in hydroponic lettuce

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