Carcinogenesis, Teratogenesis & Mutagenesis ›› 2001, Vol. 13 ›› Issue (2): 105-108.doi: 10.3969/j.issn.1004-616x.2001.02.012

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MOLECULAR ANALYSIS OF ARYLAMINE NACETYLTRANSFEASE POLYMORPHISM BY ALLELESPECIFIC PCR

YANG Ming-jing1, ZHENG Wei-juan2, XI Qing-li1, JIN Zhen-guo1   

  1. 1. Department of Biochemistry and Immunology, Jiangsu Institute of Occupational Medicine, Nanjing 210028, China; 2. Department of Biochemistry , Nanjing University, Nanjing 210008, China
  • Received:2000-06-06 Revised:2000-10-22 Online:2001-04-30 Published:2001-04-30

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Abstract: Purpose and Methods : Allele2specific PCR (AS2PCR) was modfied to identify the genotype of N-acetylt ransferase (NAT2) . Three mutations at the NAT2 gene locus , namely , 481 T , 590 A , 857 A were analyzed. We used the method to investigate the f requency of various genotypes of NAT2 in 76 unrelated Nanjing persons , and the method was tested also. Results : In the population the gene f requencies of Wt , 481 T , 590 A ,and 857 A were 0. 5723 , 0. 0395 , 0. 2368 , 0. 1513 , respectively. The f requencies for NAT2 mutations were significantly different f rom those of Whites , and were similar to those of Asian populations reported. Conclusion : The prevalence of the genotype associated with rapid acetylation among Nanjing people was 82. 9 % , and was proved that the modified AS2PCR is useful in acetylator polymorphism studies.

Key words: allele-specific PCR, polymorphism, N-acetylt ransferase

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