紫外线照射后可移植性白血病模型微核研究

doi:10.3969/j.issn.1004-616x.2001.03.007

Carcinogenesis, Teratogenesis & Mutagenesis ›› 2001, Vol. 13 ›› Issue (3): 157-159.doi: 10.3969/j.issn.1004-616x.2001.03.007

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EFFECT OF ULTRAVIOLET IRRADIATION ON THE MICRONUCLEI IN TRANSPLANTABLE LEUKEMIA MODEL

WAN Mei－rong1, CHEN Shao－zhi2

1. The Central Laboratory of A ffiliated Hospital of Xuz hou Medical College, Xuz hou 221002, China; 2.The Internal Medicine of Chil dren's Hospital of Xuz hou, Xuz hou 221005, China

Received:2000-03-02 Revised:2001-02-26 Online:2001-07-30 Published:2001-07-30

Abstract

Abstract: Purpose : To study the micronuclear changes in t ransplantable T cell leukemia model (L7212 cells) after ult raviolet irradiation. Method : Cytokinesis2block (CB) micronucleus method was used. Results : The micronucleated cell and micronucleus frequencies in the CB2blocked model ( + CB group) were higher than those in the 2CB cont rol group when the dosage was over 45 ×10 - 7 J / (s·mm2) . A dose2effect curve showed the relation ship of Y= a + blgX( P < 0. 01) . Conclusion : Cytokinesis2block micronucleus method is more sensitive than the conventional culture for the measurement of micronuclei induced by ult raviolet irradiation.

Key words: murine leukemia, L7212 cell, micronucleus, ultraviolet ray

CLC Number:

R818. 74

WAN Mei－rong, CHEN Shao－zhi. EFFECT OF ULTRAVIOLET IRRADIATION ON THE MICRONUCLEI IN TRANSPLANTABLE LEUKEMIA MODEL[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2001, 13(3): 157-159.

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EFFECT OF ULTRAVIOLET IRRADIATION ON THE MICRONUCLEI IN TRANSPLANTABLE LEUKEMIA MODEL

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