Abstract: Purpose : To observe the protective effects of the chloroform ext ract s of lsatis indigotica leaves onn the male germ cells f rom damage induced by cyclophosphamide (CP) in mice. Methods : The spermatozoon deformity rate and micronucleus rate of early spermatids were detected using spermatozoon deformity test and micronucleus test respectively. Results : Isatis indigotica had no genotoxic effect s , but showed significantly protective effect s to germ cell f rom damage induced by CP. The inhibited rates of spermatid micronucleus and spermatozoon deformity were 57. 38 % and 50. 87 % respectively at the dose of 100 mg/ kg + CP 10 mg/ kg. Conclusion : Isatis indigotica can diminish germ cell damage inducded by CP in mice.

Key words: Isatis indigotica Fort, spermatozoon malformation, micronucleus