CHO细胞体外微核高内涵筛选方法的建立及应用

doi:10.3969/j.issn.1004-616x.2011.01.008

›› 2011, Vol. 23 ›› Issue (1): 31-034.doi: 10.3969/j.issn.1004-616x.2011.01.008

• 论著 • Previous Articles Next Articles

Evaluation of micronucleus with CHO cells using high throughput screening

ZHOU Fei,LIN Hai-xia, CHANG Yan*

National Shanghai Center for New Drug Safety Evaluation and Research, Shanghai

Received:2010-05-28 Revised:2010-10-25 Online:2011-01-30 Published:2011-01-30
Contact: CHANG Yan

Abstract

Abstract: To examine the value of micronucleus high throughput screening method in micronucleus assay and develop an effective, economical, simple and fast micronucleus high throughput screening method. METHODS: To evaluate micronucleus rate with CHO cells using AssayScan High Throughput Screening system. RESULTS: B[α]P could induce micronucleus formation significantly at 25.15 μg/ml and 50.3 μg/ml. Etoposide significantly induced micronucleus formation between 0.16 μg/ml and 20 μg/ml concentrations; between 1.25 μg/ml and 20 μg/ml, cell viability was less than 50%. 2-anthramine could significantly induce micronucleus formation at 6.44 μg/ml, 12.88 μg/ml, 25.75 μg/ml and 51.5 μg/ml. Colchicine significantly induced micronucleus formation between 0.08 μg/ml and 20.4 μg/ml. EMS significantly induced micronucleus formation at 0.754 mg/ml, 1.509 mg/ml and 3.018 mg/ml. Chromium Chloride significantly induced micronucleus formation at 2.344 μg/ml. Whilst MMS could significantly induce micronucleus formation at 0.101 mg/ml. CONCLUSION: Micronucleus high throughput screening method could satisfy the requirement for an effective, economical, reliable and fast screening method.

Key words: micronucleus, high throughput screening, CHO cell, genotoxicity

ZHOU Fei,LIN Hai-xia, CHANG Yan*. Evaluation of micronucleus with CHO cells using high throughput screening[J]. , 2011, 23(1): 31-034.

[1]	YANG Yu, HUANG Yali, LIN Fei, TANG Long. Acute toxicity and genotoxicity of antitumor serum thymic factor 9 peptide [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2022, 34(1): 57-61.
[2]	DU Xiuming, HONG Liling, XU Lingzhi, ZHOU Qingyun. In vitro chromosome aberration evaluation of anatase titanium dioxide nanoparticles [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2022, 34(1): 67-71.
[3]	CAI Qi, GAO Deyu, SHI Jianfeng, YU Hao, HAN Yan, YANG Liu, ZHAO Lei, WANG Zhaoxu. Potential use of tree shrew as an animal model for the micronucleus test [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(5): 370-374,382.
[4]	LU Xue, TIAN Xuelei, ZHAO Hua, CAI Tianjing, TIAN Mei, LIU Qingjie. Use of the micronucleus assay to estimate partial radiation exposure dose in vitro [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(3): 193-196.
[5]	ZHAO Hua, LU Xue, CAI Tianjing, TIAN Mei, LIU Qingjie. Effects of age and gender on formation of nucleoplasmic bridges in peripheral blood lymphocytes [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(1): 48-52.
[6]	ZHOU Changhui, HAN Tianjiao, HUANG Pengcheng, MA Jing, CHANG Yan. Validation study of comet assay in multiple organs of rats [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(3): 233-237.
[7]	CAO Yiyi, LIU Weiying, YOU Xinyue, XI Jing, Chen Ruixue, ZHANG Xinyu, LUAN Yang. In vitro genotoxicity evaluation of BDE-3, BDE-47 and BDE-209 [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(1): 15-20,28.
[8]	WANG Quankai, XIE Guangyun, MA Shunpeng, GUO Haoran, WUHAN Baolier, SONG Jiayang, XU Jianning. Genotoxicity evaluation of glycidyl methacrylate [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(6): 479-482.
[9]	TANG Jiao, YANG Ying, LI Qing, ZHAO Min, YANG Yingfen. In vitro induction of micronuclei by Panax notoginseng extracting solution [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(5): 397-400.
[10]	LI Ying, CHEN Xiaming, LI Hanrong, LUO Te. Genotoxicity of three kinds of electric mosquito repellents on plant root tip cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(5): 401-405,411.
[11]	WEN Hairuo, GUO Yajuan, HUANG Zhiying, WANG Xue, DAN Mo. Induction of cytotoxicity and DNA damage by iron oxide nanoparticles with different surface modifications in A549 cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(6): 413-421.
[12]	XIA Ying, FU Shaohua, ZHANG Yinjing, WANG Wei. Genetic toxicity test of a pesticide, Paichongding [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(2): 136-139.
[13]	CAO Yiyi, XI Jing, TANG Weifeng, YOU Xinyue, LIU Weiying, LUAN Yang. Genotoxicity evaluation of triclosan in vitro [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(1): 71-75.
[14]	WANG Zhi, LING Xi, ZHANG Guowei, GAO Jianfang, ZOU Peng, JING Che, LIN Zhetao, LIU Jinyi, CAO Jia, AO Lin. Effect of folic acid on DNA demethylation and chromosomal damage in mice after their inhalation exposure to 1, 3-butadiene [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(3): 189-193.
[15]	MENG Xiaoqing, FANG Zhijia, WEI Hongyan, YANG Zhanju, HUANG Zhiwei. Genotoxicity investigation of tri- and hexavalent chromium using a yeast SUP4-o mutation assay [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(1): 65-69.

Evaluation of micronucleus with CHO cells using high throughput screening

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments