细菌回复突变试验菌株鉴定方法的比较

doi:10.3969/j.issn.1004-616x.2024.01.010

Abstract

Abstract: OBJECTIVE: Compare and analyze the identification methods of test strains in bacterial reverse mutation assays(Ames test) for food, medical devices, cosmetics, chemicals, pesticides and drugs in China, in order to provide references for strain identification methods. METHODS: According to the requirements of national and the profession standard, technical standards and guidelines related to Ames tests, the identification methods of histidine auxotrophy, lipopolysaccharide barrier defect(rfa mutation), ampicillin resistance(R factor), tetracycline resistance(pAQ1 plasmid) and uvrB repair deficiency(sensitivity to ultraviolet light) were analyzed and compared. RESULTS: The results showed that TA97a, TA98, TA100, TA102 and TA1535 strains all required histidine for growth, all had rfa mutations, all had ampicillin resistance except TA1535, and all were sensitive to ultraviolet light and all had no tetracycline resistance except TA102.CONCLUSION: Although the identification methods were different, the criteria and identification results were the same. Each laboratory should specify the method and frequency of identification of test strains to provide fundamental guarantee for the authenticity and reliability of Ames test results.

Key words: bacterial reverse mutation test, in vitro, genotoxicity, strain identification

CLC Number:

GAO Mei, TANG Liansheng, ZHENG Zhiyong, MA Hui, QIN Chunxue, CAO Chong. Comparison of methods to identify test strains in bacterial reverse mutation assays[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2024, 36(1): 58-65.

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Comparison of methods to identify test strains in bacterial reverse mutation assays

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