Carcinogenesis, Teratogenesis & Mutagenesis ›› 2007, Vol. 19 ›› Issue (6): 457-459.doi: 10.3969/j.issn.1004-616x.2007.06.008

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Assessment of Mutagenicity of PBDE_47 in Human Neurobla_stoma SH_SY5Y Cells in Vitro

XU Ba-yi, HE Wei-hong, HE Ping, XIA Tao,CHEN Xue-min，WANG Ai-guo   

  1. MOE Key Lab of Environment and Health, Department of Occupational ＆ Environmental Health，School of Public Health，Tongji Medical College，Huazhong University of Science ＆ Technology
  • Received:2007-01-29 Revised:2007-05-17 Online:2007-11-30 Published:2007-11-30
  • Contact: WANG Ai-guo

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Abstract: BACKGROUND ＆ AIM: To study the mutagenicity of 2,2＇,4,4＇_tetrabromodiphenyl ethers (PBDE_47) in human neuroblastoma SH_SY5Y cells in vitro. MATERIALS AND METHODS: The frequencies of micronuclei(MNi) and nucleoplasmic bridges (NPBs) were measured after SH_SY5Y cells were exposed to different doses of PBDE_47 for 24 h in vitro. RESULTS: PBDE_47 caused a significant concentration_dependent decrease in nuclear division index(NDI), and a concentration_dependent increase in MNi frequency in terms of total MNi/1000 binucleated cells (BNCs), BNCs with MNi/1000 BNCs, and NPBs/1000 BNCs. The NDI was obviously decreased, while the frequencies of BNCs with MNi/1000 BNCs and NPBs/1000 BNCs were increased significantly at 2 μg/ml and above PBDE_47 _treated groups (P<0.05). There was statistically difference in frequcency of MNi/1000 BNCs only in 4 μg/ml PBDE_47_treated group (P<0.05). CONCLUSION: PBDE_47 may induce the MNi and NPB formation, causing mutagenicity.

Key words: PBDE_47, mutagenicity, micronuclei, nucleoplasmic bridges