Abstract: BACKGROUND AND AIM: Oxidative stress effect induced by cooking oil fume particulate (COFP) on human embryonic diploid lung fibroblasts (HELF)was investigated. MATERIALS AND METHODS: Glass fibre filter film was used to collect COFP. IC50 was determined by MTT assay. HELF cells were exposed to COFP at the doses 20、4、0.8 μg/ml for 12, 24 and 48 hours. Then ROS analysis, MDA test and comet assay were performed. RESULTS: The IC50 for 12, 24, 48 hours was 101.7 μg/ml, 82.7 μg/ml and 85.1 μg/ml, respectively. The mean cytoplasmic fluorescence intensity increased as the COFP dose increased. Significant difference in the mitochondria was observed between 0.8 and 4 μg/ml dose groups and negative control group for 24 and 48 hours exposure duration. No significant difference was observed between treated groups and negative control group for MDA test. However, there was significant difference between treated groups and negative control group for comet assay. No significant difference was observed among treated groups for different exposure time in all the assays. CONCLUSION: In the range of experimental dosage and exposure duration, ROS increase in both cytoplasmic and mitochondria and DNA damage were observed, while peroxidation of lipid was not evident.

Key words: cooking oil fume particuate, oxidative stress, ROS, malondialdehyde, DNA breakage