Carcinogenesis, Teratogenesis & Mutagenesis ›› 2008, Vol. 20 ›› Issue (2): 123-126.doi: 10.3969/j.issn.1004-616x.2008.02.012

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Genotoxicity of Chlorothalonil and Cypermethrin on Vicia Faba Root Tip Cells

BU Ning1 ,ZHANG Jun2 ,WANG Sheng-hou1 ,MA Lian-ju1,   

  1. 1.College of Chemistry and Life Science, Shenyang Normal University, Shenyang 110034; 2. Shenyang Agriculture Testing Center, Shenyang 110034,China
  • Received:2007-09-03 Revised:2007-09-21 Online:2008-03-30 Published:2008-03-30
  • Contact: MA Lian-ju

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Abstract: BACKGROUND AND AIM: The genotoxicity of chlorothalonil or cypermethrin on vicia faba root tip was studied by using micronucleus test technology. MATERIALS AND METHODS: Micronucleus assay and chromosomal aberration assay were used to determine the mitotic index, the micronucleus frequency and chromosomal aberration frequency of Vicia faba root tip cells induced by different concentrations of chlorothalonil and cypermethrin. RESULTS: Chlorothalonil and cypermethrin could increase the micronucleus rate of vicia faba root tip cells. Within a certain concentration range(5×10－10－5×10－6 g/L chlorothalonil, 5×10－10－5×10－8 g/L cypermethrin), the rate of micronucleus increased with increasing concentration of chlorothalonil or cypermethrin, but decreased with further increase in concentrations. The cell mitosis index increased in different concentrations of chlorothalonil or cypermethrin. Besides, various types of chromosome aberration were also induced. CONCLUSION: Chlorothalonil and cypermethrin could produce apparent genotoxicity on Vicia faba root tip.

Key words: vicia faba, chlorothalonil, cypermethrin, micronucleus frequency, mitotic index, chromosome aberration frequency