鳞盖肉齿菌的抗肿瘤细胞增殖活性及其机制的研究

doi:10.3969/j.issn.1004-616x.2006.05.015

Carcinogenesis, Teratogenesis & Mutagenesis ›› 2006, Vol. 18 ›› Issue (5): 387-389.doi: 10.3969/j.issn.1004-616x.2006.05.015

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Anti-tumor Effects and Mechanisms of Sarcodon Scabrosus Karst Extracts

WANG Gang1 ,GUO Wen-zhi2, SUN Zhuo2, HE Dong-wei2，Hitota Mituru3, DONG Mei2,

1. Department Pharmacy Engneer, Hebei Chemical ＆ Pharmaceutical Vocational Technology College. Shijiazhuang 050031, Hebei, China; 2. Faculty of Forensic Medicine, the School of Basic Medical Sciences, Hebei Medical University, Shijiazhuang 050017;3. Department of Bioscience and Biotechnology, Faculty of Agriculture, Shinshu University, Nagano 399－4598 Japan

Received:2006-01-17 Revised:2006-03-22 Online:2006-09-30 Published:2006-09-30
Contact: DONG Mei

Abstract

Abstract: BACKGROUND＆AIM: To study the anti-tumor effects of Sarcodonin G, extracted from Sarcodon scabrosus karst by dichlorolomethane on HeLa cell in vitro and explore the possible mechanisms. MATERIAL AND METHODS: The effect of Sarcodonin G on HeLa cell line was determined by MTT assay in vitro. The apoptosis induced by Sarcodonin G was studied by FCM. The morphological changes of HeLa cells were assessed by transmission electron microscope. RESULTS：Sarcodonin G dose dependently inhibited the proliferation of cultured HeLa cells, the IC50 was 7.19 μmol/L. Compared with the control group, the apoptosis rate and the expression of P53 in HeLa cells were markedly higher in the three Sarcodonin G group(P<0.01). The morphological changes of HeLa cells incubated with Sarcodonin G showed chromatin condensation, marginal and nuclear fragmentation. CONCLUSION: Sarcodonin G could inhibit the proliferation of HeLa cells in vitro. Its anti-tumor effect might be associated with up-regulating the expression of P53 and inducing apoptosis.

Key words: sarcodon scabrosus karst, sarcodonin G, snti-tumor, apoptosis, P53

WANG Gang , GUO Wen-zhi, SUN Zhuo, HE Dong-wei, Hitota Mituru, DONG Mei, . Anti-tumor Effects and Mechanisms of Sarcodon Scabrosus Karst Extracts[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2006, 18(5): 387-389.

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Anti-tumor Effects and Mechanisms of Sarcodon Scabrosus Karst Extracts

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