pEgr-MDA7重组质粒辐射诱导特性的检测

doi:10.3969/j.issn.1004-616x.2006.05.013

Carcinogenesis, Teratogenesis & Mutagenesis ›› 2006, Vol. 18 ›› Issue (5): 381-383.doi: 10.3969/j.issn.1004-616x.2006.05.013

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The Irradiation-Induced Property of pEgr-MDA7 Recombinant Plasmid

WU Cong-mei1, HUANG Tian-hua1, Wu De-sheng1,XIE Qing-dong1,XU Xiao-hu2

1.Research Center of Reproductive Medicine,Shantou University Medical College, Shantou 515041, Guangdong,China;2.Department of Forensic Medicine,Shantou University Medical College,Shantou 515041,Guang Dong,China

Received:2005-01-28 Revised:2005-04-20 Online:2006-09-30 Published:2006-09-30
Contact: WU Cong-mei

Abstract

Abstract: BACKGROUND ＆ AIM: To clone human MDA7 cDNA gene, construct pEgr-MDA7 plasmid, and measure its mRNA level in esophageal squamous EC9706 cells induced by irradiation. MATERIAL AND METHODS: Human MDA7 cDNA gene was isolated from peripheral blood and was then ligated to downstream of Egr-1 promoter to construct pEgr-MDA7 plasmid. The recombinant plasmids were transfected into EC9706 cells with liposome, then MDA7 mRNA level of after different doses of X-ray irradiation was quantified by PCR. RESULTS: Sequencing and restriction enzyme digestion were performed ensuring that MDA7 cDNA gene cloning and construction of pEgr-MDA7 were correct. MDA7 mRNA level in cells transfected with pEgr-MDA7 induced by different doses of irradiation was higher than that of sham-irradiation group. CONCLUSION: X-ray could induce and enhance expression of pEgr-MDA7 gene in EC9706 cells.

Key words: melanoma differentiation associated gene, pEgr-MDA7 recombinant plasmid, irradiation

WU Cong-mei, HUANG Tian-hua, Wu De-sheng, XIE Qing-dong, XU Xiao-hu. The Irradiation-Induced Property of pEgr-MDA7 Recombinant Plasmid[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2006, 18(5): 381-383.

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The Irradiation-Induced Property of pEgr-MDA7 Recombinant Plasmid

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