五种氯化有机物对V79细胞DNA 损伤作用的研究

doi:10.3969/j.issn.1004-616x.1999.01.002

Carcinogenesis, Teratogenesis & Mutagenesis ›› 1999, Vol. 11 ›› Issue (1): 5-008.doi: 10.3969/j.issn.1004-616x.1999.01.002

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STUDY ON THE DNA DAMAGE OF FIVE CHLORINATED ORGANIC COMPOUNDS IN V79 CELLS

Zhang Zunzhen1 ,Heng Zheng Chang1 ,Li Nan2 ,La Bazuoma2

1Department of Envi ronmental Heal th , West China University of Medical Sciences , Chengdu 610041 2Students of Preventive Medicine

Received:1998-06-08 Revised:1998-08-14 Online:1999-01-30 Published:1999-01-30

Abstract

Abstract: In this paper , the DNA damage effect s of carbon tet rachlororide , t richloromethane ,dichloromethane , chloroacetic acid and t richloroacetic acid were studied using the single cell gel elect rophoresis technique ( comet assay) in V79 cells cultured in vit ro. The result s showed that all test substances , except trichloroacetic acid , exhibited a clear and reproducible linear dose2dependent increase in DNA migrtion. The DNA breakage capacity and the cytotoxicity of this chemicals have the same order : carbon tet rachlororide >t richloromethane > dichloromethane > chloroacetic acid > t richloroacetic acid. The data also suggested that DNA breakage capacity and the cytotoxicity of the chemicals of chlorinated alky1 were st ronger than that of the kind of chloroacetic acid. Among the chemicals of chlorinated alky1 , the DNA breakage capacity increased with increasing in the number of chlorine substitution.

Key words: Chlorinated organic compound, Single cell gel elect rophoresis, DNA damage, V79 cell

Zhang Zunzhen , Heng Zheng Chang , Li Nan , La Bazuoma. STUDY ON THE DNA DAMAGE OF FIVE CHLORINATED ORGANIC COMPOUNDS IN V79 CELLS[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 1999, 11(1): 5-008.

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STUDY ON THE DNA DAMAGE OF FIVE CHLORINATED ORGANIC COMPOUNDS IN V79 CELLS

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