肉苁蓉苯乙醇总苷对大鼠肝星状细胞TGF-β1/Smad信号通路表达的影响

doi:10.3969/j.issn.1004-616x.2016.05.008

Carcinogenesis, Teratogenesis & Mutagenesis ›› 2016, Vol. 28 ›› Issue (5): 372-376.doi: 10.3969/j.issn.1004-616x.2016.05.008

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Effect of phenylethanol glycosides on the TGF-β1/Smad signaling pathway in the rat HSC-T6 hepatic stellate cells

YOU Shuping¹, ZHAO Jun², MA Long¹, ZHANG Shilei¹, LIU Tao¹

1. Department of Toxicology, College of Public Health, Xinjiang Medical University, Urumqi 830011;
2. Xinjiang Uygur Autonomous Region Institute of Materia Medica, Urumqi 830004, Xinjiang, China

Received:2016-04-13 Revised:2016-06-23 Online:2016-09-30 Published:2016-09-30

Abstract

Abstract: OBJECTIVE: To investigate the effect of phenylethanol glycosides from cistanchetubulosa(CPhGs) on cell proliferation and activation of TGF-β1-induced hepatic stellate cells (HSC-T6). METHODS: HSC-T6 cells were cultivated with CPhGs in different concentrations (25,50,75,100 μg/mL). After the cells were stimulated with TGF-β1,cell activity and drug cytotoxicity were determined by MTT and LDH,respectively. Smad2,Smad3,Smad7 mRNA and Smad2,Smad3,p-Smad2,p-Smad3,Smad7 protein expressions were assayed by qPCR and Western blot. RESULTS: CPhGs at 50-100 μg/mL concentrations effectively inhibited TGF-β1-mediated cell proliferation (P<0.05) and CPhGs at 25-100 μg/mL concentrations did not induce significant cytotoxicity. CPhGs at 25-100 μg/mL concentrations,inhibited Smad2 and Smad3 mRNA and protein levels,as well as the phosphorylation levels of Smad2 and Smad3 proteins,but increased Smad7 mRNA and protein expression significantly on HSC-T6 (P<0.05 or P<0.01). CONCLUSION: CPhGs demonstrated the protective effect against hepatic fibrosis. The mechanism of this process could involve interference with the TGF-β1/Smad signaling pathway,and inhibition of activation and proliferation of HSC-T6.

Key words: phenylethanol glycosides from cistanchetubulosa, transforming growth factor-β1, hepatic stellate cells, hepatic fibrosis

CLC Number:

R961.1

YOU Shuping, ZHAO Jun, MA Long, ZHANG Shilei, LIU Tao. Effect of phenylethanol glycosides on the TGF-β1/Smad signaling pathway in the rat HSC-T6 hepatic stellate cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2016, 28(5): 372-376.

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Effect of phenylethanol glycosides on the TGF-β1/Smad signaling pathway in the rat HSC-T6 hepatic stellate cells

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