线粒体DNA中D-loop区突变在非小细胞肺癌中的意义

doi:10.3969/j.issn.1004-616x.2013.02.011

Abstract

Abstract:

OBJECTIVE: To study the role of mitochondrial DNA (mtDNA) mutations in D-loop region in non-small cell lung cancer patient’s and its possible clinical application. METHODS：The mtDNA of both cancer and paracancerous tissue cells of 20 non-small cell lung cancer patients was extracted， the D-loop region propagated by PCR sequenced，and mutations taking the mtDNA D-loop sequences of the human genome as control were analyzed. RESULTS：Mutations were found in the sequence of the cancer and paracancerous tissue cells of all 20 cases of non-small cell lung cancers. Among the 265 mutations there were 69 specific mutations of cancer tissue，96 specific mutations of paracancerous tissue and 50 common mutations. CONCLUSION：The mutations of D-loop region of mtDNA occurred mainly in HVRII region，and it may play an important role in the development of non-small cell lung cancer.

Key words: mtDNA, D-loop region, mutation, lung cancer

JIANG Lei1，WANG Zu-yi1，*，WANG Guo-wen1，ZHAO Hua2. The significance of mitochondrial DNA D-loop region mutations in non-small cell lung cancer[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2013.02.011.

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The significance of mitochondrial DNA D-loop region mutations in non-small cell lung cancer

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