Carcinogenesis, Teratogenesis & Mutagenesis

Previous Articles     Next Articles

Effects of (-)epigallocatechin gallate on expressions of hMLH1 and hMSH2

NI Juan1LI Zhen1,XUE Jing-lun2,WANG Xu1,*   

  1. 1. School of Life Sciences, Yunnan Normal University, Engineering Research Center of Sustainable Development and Utilizaton of Biomass Energy, Ministry of Education, Kunming 650500, Yunnan; 2. Institute of Genetics, Fudan University, Shanghai 200433, China
  • Received:2013-02-21 Revised:2013-04-29 Online:2013-07-30 Published:2013-07-30
  • Contact: WANG Xu,E-mail:wangxu@fudan.edu.cn

Abstract:

OBJECTIVE: To investigate the effect of (-) epigallocatechin gallate(EGCG) on mRNA expressions of hMLH1 and hMSH2 in human lymphoblast cell lines. METHODS: Total RNA was isolated from GM12593 and GM13705 cells treated with EGCG(0,5,10,20 μmol/L) for 6 days. Real-time fluorecence quantification PCR(FQ-PCR) was conducted to measure the mRNA expression levels of hMLH1 and hMSH2. RESULTS: The mRNA expressions of hMLH1 and hMSH2 significantly increased at 20 μmol/L EGCG in GM12593 cells,and were significantly higher than equal concentrations in GM13705 cells. The effect of EGCG on expressions of target genes were insignificant in GM13705 cells. CONCLUSION: EGCG had the potential to up-regulate the expressions of hMLH1 and hMSH2, contributed to the promotion of mismatch repair and maintaining genomic stability in normal human lymphoblast cell line GM12593.

Key words: (-)epigallocatechin gallate, human lymphoblast cell line, mismatch repair gene, gene expression