Carcinogenesis, Teratogenesis & Mutagenesis

Previous Articles     Next Articles

The effect of combining FOLFOX4 with 1-D-methyl tryptophan on regulatory T cells of gastric cancer-bearing mice

KANG Gui-yun1LI Zhe-ping1,LIU Xiao-li2,WU Xi-run2,SHEN Hui-qin2,WANG Qi2,*   

  1. 1. Graduate School, Shanxi Medical University, Taiyuan 030001, Shanxi; 2. Department of Gastroenterology, Second Hospital of Shanxi Medical University, Taiyuan 030001, Shanxi, China
  • Received:2013-03-29 Revised:2013-05-15 Online:2013-07-30 Published:2013-07-30
  • Contact: WANG Qi,


OBJECTIVE: To investigate whether combining 1-D-MT with FOLFOX4 could be useful to improve the immune tolerance of gastric cancer- bearing mice. METHODSBy using the lipofectamineTM 2000,the eukaryotic expression plasmid pcDNA3.1-IDO and empty vector pcDNA3.1 (+) were transfected in a MFC cell line,setting a control group. The expression of IDO was detected by reverse transcription polymerase chain reaction (RT-PCR) and western blot. Animal model of gastric cancer- bearing mice were established to receive normal saline (NS),FOLFOX4,1-D-MT and 1-D-MT+FOLFOX4 therapy. By using flow cytometry,Treg cell ratio change was analyzed and FOXP3 mRNA expression change was assessed by RT-PCR. RESULTS:The expression of IDO mRNA and protein in the group trancfected with pcDNA3.1-IDO was obviousely ligher than the MFC group and the empty vector group (P<0.05). Treg cell ratio and FOXP3 mRNA expression in the transfected IDO negative control group with NS therapy was higher than the MFC and the empty vector groups (P<0.05). The Treg ratio and FOXP3 mRNA in 1-D-MT+FOLFOX4 and 1-D-MT groups were less than in FOLFOX4 group (P<0.05),with 1-D-MT+FOLFOX4 group more markedly than 1-D-MT group (P<0.05). CONCLUSION:Combining 1-D-MT with FOLFOX4 could reduce Treg cell ratio and FOXP3 expression,thus reducing the immune escape.

Key words: 1-D-methyl tryptophan, FOLFOX4, gastric cancer, regulatory T cell