姜黄素对HaCaT细胞凋亡过程中相关基因表达的影响

doi:10.3969/j.issn.1004-616x.2014.04.003

Abstract

Abstract:

OBJECTIVE: To explore the effects of curcumin on apoptosis-related gene expression in HaCaT cells. METHODS：Immunocytochemistry and Western blot were used to detect the effects of curcumin on apoptosis-related protein expression. RESULTS：Immunocytochemistry staining results showed that the expression levels of P53，Bax，Fas were upregulated significatntly while the products of Bcl-2 proteins were downregulated significantly in cells treated with 7.5 μg/mL curcumin. Western blot analysis confirmed the same results. CONCLUSION：Curcumin induced apoptosis of HaCaT cells by inhibiting expression of Bcl- 2 gene and promoting the apoptosis genes p53，Bax and Fas activation. Curcumin could induce apoptosis by regulation of a variety of apoptosis-related genes to activate the apoptosis signaling pathway.

Key words: HaCaT cells, cell apoptosis, curcumin, apoptosis-related genes

SONG Wei，YANG Hai-bo，CHEN Lan-ying*. Effects of curcumin on related gene expression during HaCaT cell apoptosis[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2014.04.003.

References

[1] Stein M，Bernd A，Ramirez-Bosca A，et al. Measurement of anti-inflammatory effects of glucocorticoids on human keratinocytes in vitro. Comparison of normal human keratinocytes with the keratinocyte cell line HaCaT[J]. Arzneimittel-Forschung，1997，47(11)：1266-1270.

[2] MY L，YG W，JX W，et al. Curcumin inhibits 19-kDa lipoprotein of mycobacterium tuberculosis induced macrophage apoptosis via regulation of the JNK pathway[J]. Biochem Biophys Res Commun，2014，446(2)：626-632.

[3] Xu MX，Zhao L，Deng C，et al. Curcumin suppresses proliferation and induces apoptosis of human hepatocellular carcinoma cells via the wnt signaling pathway[J]. Int J Oncol，2013，43(6)：1951-1959.

[4] Wang WZ，Li L，Liu MY，et al. Curcumin induces FasL-related apoptosis through p38 activation in human hepatocellular carcinoma Huh7 cells[J]. Life Sci，2013，92(6)：352-358.

[5] Shehzad A，Lee J，Huh TL，et al. Curcumin induces apoptosis in human colorectal carcinoma (HCT-15) cells by regulating expression of Prp4 and p53[J]. Mol Cells，2013，35(6)：526-532.

[6] Yang CW，Chang CL，Lee HC，et al. Curcumin induces the apoptosis of human monocytic leukemia THP-1 cells via the activation of JNK/ERK pathways[J]. BMC Complement Altern Med，2012，12(1)：22-30.

[7] Herr I，Debatin KM. Cellular stress response and apoptosis in cancer therapy[J]. Blood，2001，98(9)：2603-2614.

[8] Li Y，Zhang S，Geng JX，et al. Curcumin inhibits human non-small cell lung cancer A549 cell proliferation through regulation of Bcl-2/Bax and cytochrome C[J]. Asian Pac J Cancer Prev，2013，14(8)：4599-4602.

[9] Yu J，Zhou X，He X，et al. Curcumin induces apoptosis involving bax/bcl-2 in human hepatoma SMMC-7721 cells[J]. Asian Pac J Cancer Prev，2011，12(8)：1925-1929.

[10] Ghate NB，Hazra B，Sarkar R，et al. Alteration of Bax/Bcl-2 ratio contributes to Terminalia belerica-induced apoptosis in human lung and breast carcinoma[J]. In Vitro Cell Dev Biol Anim，2014：1-11.

[11] Jacobson MD. Apoptosis：Bcl-2-related proteins get connected [J]. Curr Biol，1997，7(5)：R277-R281.

[12] Symonds H，Krall L，Remington L，et al. p53-dependent apoptosis suppresses tumor growth and progression in vivo[J]. Cell，1994，78(4)：703-711.

[1]	QIN Shuangjian, LI Boru, CAI Ying, ZHENG Kai, WANG Bingyu, LI Runbing, XIAO Fang, ZENG Ming, XU Xinyun. PM_2.5 exposure on expression of oncogenes and apoptosis genes in hepatocytes [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(4): 281-285.
[2]	LI Peng, MA Kun, WU Haoyu, WU Yanping, LI Baixiang. Mechanism of isoflavones protection of SH-SY5Y cells from atrazine [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(1): 25-30.
[3]	ZHANG Chaoning, LI Jintian, LIU Yongqi, LIN Xingyao. Expression of apoptosis-related genes in lung and kidney tissues of rats with radiation-induced bystander effects [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(1): 42-46.
[4]	ZHOU Qiming, LI Wei, YUAN Jianhui, ZHAO Yan, DUAN Jiangman, XIONG Donglin. Abnormal expression of TGF-β1 in melanoma patients and its inhibitory effect on tumor cell proliferation [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2016, 28(5): 348-352.
[5]	LI Chen, QI Xuesong, LI Ning, FAN Li, GOU Qiao, LIU Jianxiang, TIAN Mei. UVA ultraviolet radiation on cellular injury to human keratinocytes [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2016, 28(5): 364-368.
[6]	FU Ying, ZHAO Chen, CHANG Zhenzhan, LIN Huaying, SUN Zhenxiao. Inhibition effects of Arca subcrenata Lischke anticancer protein on human breast carcinoma MCF-7 cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2015, 27(6): 415-420.
[7]	YIN Hua, LI Wenxue, LI Mengcheng, DENG Yuting, LI Jinhui, LI Juntao, WU Jinyin, YANG Guangyu, LIN Zhongning, ZHU Wei. Preventive effect and mechanisms of 3,3'-diindolylmethane on oxidative stress induced by hydrogen peroxide in HaCaT cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2015, 27(2): 81-85,90.
[8]	ZHENG Fuchun, CHEN Yicun, QIN Yongjie, CAI Congyi. Anti-inflammatory activity of chemical components from n-butanol extract of Ipomoea stolonifera [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2015, 27(2): 146-149,153.
[9]	YANG Hai-bo，SONG Wei，CHEN Lan-ying. Effect of curcumin on proliferation of human bile duct carcinoma MZ-Cha-1 cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2013, 25(4): 289-292.
[10]	ZHANG Wei;WANG Ling;XU Pei-yu;XIAO Heng-yi. Synergistic tumor suppression by curcumin and low-concentration vincristine in hepatoma cell lines [J]. , 2012, 24(4): 270-274,.
[11]	XIONG Meng-zhen，ZHANG Juan，SUN Rong-li，YIN Li-hong，PU Yue-pu. Apoptotic effect and regulatory mechanism of 1, 4-benzoquinone on HELF cells [J]. , 2012, 24(1): 6-9.
[12]	ZHAO Wen-hong1,ZHUANG Ying1,ZHAO Hong1,ZHANG Ding2. Human Umbilical Vein Endothelial Cell Apoptosis and Its Proliferative Inhibition Induced by Cholesterin [J]. , 2007, 19(6): 453-456.
[13]	LI Run_qing, SHAN Bao_en. Effects of Quercetin on Proliferation, Cell Cycle and Cyclin B1 Protein Expression of Colon Carcinoma Cell Line SW480 [J]. , 2007, 19(5): 384-387.
[14]	ZHANG Jing1; SHAN Bao-en1; LIU Gang-san2; ZHAO Xue-tao1; CHEN Shu-hong2. Anti-tumor Activity of Extract from Periploca Sepium Bge [J]. , 2006, 18(2): 108-111.
[15]	SHAN Bao-en; GUO Lan-tao; DONG Qing; MA Hong. Syk Can Influence As2O3 Induced Brain Tumor Cell Cycle Defects [J]. , 2005, 17(6): 346-349.

Effects of curcumin on related gene expression during HaCaT cell apoptosis

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments