欧前胡素对体外培养的人表皮黑素细胞Rab27a和酪氨酸酶的作用

doi:10.3969/j.issn.1004-616x.2014.04.009

Abstract

Abstract:

OBJECTIVE: To investigate the effects of imperatorin on Rab27a and tyrosinase in cultured human epidermal melanocytes. METHODS：Human epidermal melanocytes were separated，purified and cultured in vitro. Melanocytes were randomly divided into control group and imperatorin group. After 48 h incubation with culture medium M254 or imperatorin(50 or 25 μmol/L)，the morphology of melanocytes was examined by inverted phase contrast microscopy. The effects of imperatorin on Rab27a and tyrosinase in melanocytes were investigated by immunofluoresent assay. The ultrastructures of melanocytes and melanosomes were studied by transmission electron microscopy. RESULTS：The melanocytes cultured with imperatorin for 48 h had shorter dendrites. Immunofluorescence microscopy showed that the location of Rab27a and tyrosinase in melanocytes were not obviously changed，but the fluorescence intensity of Rab27a was enhanced (P<0.05) and tyrosinase attenuated (P<0.05). Fewer melanosomes found in the cultured human epidermal melanocytes by transmission electron microscopy. CONCLUSION：Imperatorin promoted the expression of Rab27a and inhibited tyrosinase activity.

Key words: melanocyte, imperatorin, tyrosinase, Rab27a, melanosome, transfer

XIE Jia-jun，LIN Ling，LIU Shao-gang，WU Xiao-jia，RUAN Hai-dong，GUO Pei-xiang，TONG Peng-fei，FANG Bin，CHEN Wei-ying，SHE Shao-yi. Effects of imperatorin on Rab27a and tyrosinase in cultured human epidermal melanocytes[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2014.04.009.

References

[1] Strom M，Hume AN，Tarafder AK，et al. A family of Rab27-binding proteins. Melanophilin links Rab27a and myosin Va function in melanosome transport[J]. J Biol Chem，2002， 277(28)：25423-25430.

[2] 雷铁池，朱文元，夏明玉，等. 中药对黑素生物合成影响研究I 82味中药乙醇提取物对酪氨酸酶活性的抑制作用[J]. 中草药，1999，30(5)：336－339.

[3] 王卫，魏静. 中医中药治疗黄褐斑246例临床观察[J]. 中华现代中医学杂志，2006，2(3)：270－271.

[4] 陈玮莹，宾向玲，蔡绍先，等. 七白膏方剂中药提取物对离体人黑素细胞的作用[J]. 中国麻风皮肤病杂志，2011， 27(6)；390-393.

[5] Huang Q，Liang W，Xu D，et al. Ultrastructural observations of human epidermal melanocytes cultured on polyethylene terephthalate film[J]. Micron，2013，48(1)：49-53.

[6] Quast T，Wehner S，Kirfel G，et al. sAPP as a regulator of dendrite motility and melanin release in epidermal melanocytes and melanoma cells[J]. FASEB J，2003，17(12)：1739-1741.

[7] 蔡绍先，陈玮莹，吴健谊，等. 五味子对CCl4中毒大鼠肝超微结构的保护作用[J]. 癌变·畸变·突变，2010，22(1)： 52-54.

[8] Costin GE，Hearing VJ. Human skin pigmentation： melanocytes modulate skin color in response to stress[J]. FASEB J，2007，21(4)：976-994.

[9] Wu X，Rao K，Bowers MB，et al. Rab27a enables myosin Va-dependent melanosome capture by recruiting the myosin to the organelle[J]. J Cell Sci，2001，114(Sup 6)：1091-1100.

[10] 杨小花，胡晓. 欧前胡素与异欧前胡素的药理学研究进展[J]. 南昌大学学报：医学版，2012，52(3)：95-100.

[11] Hume AN，Collinson LM，Rapak A，et al. Rab27a regulates the peripheral distribution of melanosomes in melanocytes[J]. J Cell Biol，2001，152(4)：795-808.

[12] Kim B，Lee JY，Lee HY，et al. Hesperidin suppresses melanosome transport by blocking the interaction of Rab27A-Melanophilin[J]. Biomol Ther，2013，21(5)：343-348.

[13] Hume AN，Seabra MC. Melanosomes on the move：a model to understand organelle dynamics [J]. Biochem Soc Trans，2011， 39(5)：1191-1196.

[14] Ando H，Niki Y，Yoshida M，et al. Involvement of pigment globules containing multiple melanososmes in the transfer of melanosomes from melanocytes to keratinocytes[J]. Cell Logist， 2011，1(1)：12-20.

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Effects of imperatorin on Rab27a and tyrosinase in cultured human epidermal melanocytes

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