B[a]P和DDT亚急性联合暴露对小鼠肝功能酶ALT、AST和γ-GT的影响及作用形式

doi:10.3969/j.issn.1004-616x.2015.04.004

Abstract

Abstract: OBJECTIVE: To explore the effects of benzo[a] pyrene and DDT exposure alone or in combination on ALT,AST and γ-GT in mice. METHODS: Fifty healthy male Kunming mice were divided into 10 groups at random: blank control group (normal feed);solvent control group(equal volume of oil);1,10 mg/(kg·d) B[a]P groups;0.6,6 mg/(kg·d) DDT groups;1 mg/(kg·d) B[a]P+0.6 mg/(kg·d) DDT group;1 mg/(kg·d) B[a]P+6 mg/(kg·d) DDT group;10 mg/(kg·d) B[a]P+0.6 mg/(kg·d) DDT group and 10 mg/(kg·d) B[a]P+6 mg/(kg·d) DDT group. Exposure groups were treated with oil contained B[a]P and DDT once a day. After 31 days of intraperitoneal injection,eyeballs were extracted and ALT,AST and γ-GT were examined in blood. HE slices were made to confirm the liver damage. Using two factors and three levels factorial ANOVA design for data analysis. RESULTS: The levels of ALT and AST in all B[a]P or DDT alone exposure groups were significantly higher than that of the control groups (F=41.308,P=0.000;F=20.083,P=0.000),showing dose-effect relationships in both. But no interaction was observed in co-exposure groups(P=0.258,P=0.264). No significant changes in γ-GT were observed in all B[a]P or DDT alone exposure groups. Neither was there any interactive effect in co-exposure groups(P=0.816). This was in accordance with the observation in HE slice: hydropic degeneration,endolysis and nuclear enlargement in liver cell. CONCLUSION: Under this experimental condition,increasing levels of ALT and AST could be detected in B[a]P or DDT separate exposure groups. But neither could affect γ-GT level. No interactive effects of ALT,AST and γ-GT could be induced in co-exposure groups.

Key words: benzo[a]pyrene, DDT, co-exposure, ALT, AST, γ-GT, interactive effect

CLC Number:

R994.6

ZHAO Ran, LIU Min, WANG Juan, ZHENG Fang, GUO Dongbei, FAN Chun. Effects of benzo[a]pyrene and DDT subacute co-exposure on ALT, AST and γ-GT in mice[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2015, 27(4): 272-277.

References

[1] Camacho M,Luzardo OP,Boada LD,et al. Potential adverse health effects of persistent organic pollutants on sea turtles: evidences from across-sectional study on Cape Verde logger head sea turtles[J]. Sci Total Environ,2013,458/459/460: 283-289.
[2] Cok I,Mazmanci B,Mazmanci MA,et al. Analysis of human milk to assess exposure to PAHs,PCBs and organochlorine pesticides in the vicinity Mediterranean city Mersin,Turkey[J]. Environ Int,2012,40(1):63-69.
[3] Liao C,Lv J,Fu J,et al. Occurrence and profiles of polycyclic aromatic hydrocarbons (PAHs),polychlorinated biphenyls (PCBs) and organochlorine pesticides (OCPs) in soils from a typical e-waste recycling area in Southeast China[J]. Int J Environ Health Res,2012,22(4):317-330.
[4] Litskas VD,Dosis IG,Karamanlis XN,et al. Occurrence of priority organic pollutants in Strymon river catchment, Greece:inland,transitional,and coastal waters[J]. Environ Sci Pollut Res Int,2012,19(8):3556-3567.
[5] Guo Y,Yu HY,Zeng EY. Occurrence,source diagnosis, and biological effect assessment of DDT and its metabolites in various environmental compartments of the Pearl River Delta, South China:A review[J]. Environ Pollut,2009,157(6): 1753-1763.
[6] Deribe E,Rosseland BO,Borgstrøm R,et al. Organochlorine pesticides and polychlorinated biphenyls in fish from Lake Awassa in the Ethuopian Rift Valley:human health risks[J]. Bull Environ Contam Toxicol,2014,93(2):238-244.
[7] Fianko JR,Donkor A,Lowor ST,et al. Health risk associated with pesticide contamination of fish from the Densu River Basin in Ghana[J]. J Environ Prot,2011,2:115-123.
[8] Polder A,Savinova TN,Tkachev A,et al. Levels and patterns of persistent organic pollutants (POPS) in selected food items from Northwest Russia (1998-2002) and implications for dietary exposure [J]. Sci Total Environ,2010,408(22):5352-5361.
[9] Chen WQ,Hong HS,Xu L,et al. Persistent organochlorine pollutants in surface sediments and economic shellfish of Minjiang Estuary-Mazu and Xiamen-Jinmen sea areas[J]. J Environ Sci Health A Tox Hazard Subst Environ Eng,2005, 40(10):1895-1903.
[10] Qin YY,Leung CK,Lin CK,et al. Halogenated POPs and PAHs in blood plasma of Hong Kong residents[J]. Environ Sci Technol,2011,45(4):1630-1637.
[11] Guéguen M, Amiard JC, Arnich N, et al. Shellfish and residual chemical contaminants:hazards,monitoring,and health risk assessment along French coasts[J]. Rev Environ Contam Toxicol,2011,213(1):55-111.
[12] Bliss CI. The toxicity of poisons applied jointly[J]. Ann Appl Biol,1939,26:585-615.
[13] Council of the European Union. Council Conclusionson Combination Effects of Chemicals [R/OL].(2014-12-19) http: //www.consilium.europa.eu/uedocs/cms_data/docs/pressdata/en/envir/112072.pdf
[14] Tarantini A,Maitre A,Lefèbvre E,et al. Polycyclic aromatic hydrocarbons in binary mixtures modulate the efficiency of benzo[a]pyrene to form DNA adducts in human cells[J]. Toxicology,2011,279(1/2/3):36-44.
[15] Chanyshev MD,Kosorotikov NI,Titov SE,et al. Expression of microRNAs,CYP1A1 and CYP2B1 in the livers and ovaries of female rats treated with DDT and PAHs[J]. Life Sci, 2014,103(2):95-100.
[16] Wei W,Zhang C,Liu AL,et al. Effect of PCB153 on BaP-induced genotoxicity in HepG2 cells via modulation of metabolic enzymes[J]. Mut Res,2009,675(1/2):71-76.
[17] Irena H,Metka F. Organophosphorus pesticides enhance the genotoxicity of benzo(a)pyrene by modulating its metabolism[J]. Mut Res,2009,675(1/2):84-92.
[18] Wei W,Zhang C,Liu AL,et al. PCB126 enhanced the genotoxicity of BaP in HepG2 cells by modulating metabolic enzyme and DNA repair activities[J]. Toxicol Lett,2009, 189(2):91-95.
[19] Wu XJ,Lu WQ,Mersch-Sundermann V. Benzo(a)pyrene induced micronucleus formation was modulated by persistent organic pollutants (POPs) in metabolically competent human HepG2 cells[J]. Toxicol Lett,2003,144(2):143-150.
[20] 方积乾. 医学统计学与电脑实验[M]. 3版. 上海:上海科学技术出版社,2006:170.
[21] Fent K. Ecotoxicology of organotin compounds[J]. Crit Rev Toxicol,1996,26(1):1-117.
[22] Rouleau C,Gobeil C,Tjälve H. Pharmacokinetics and distribution of dietary tributyltin compared to those of methylmercury in the American plaice Hippoglossoides platessoides[J]. Mar Ecol Prog Ser,1998,171:275-284.
[23] Denison MS,Nagy SR. Activation of the aryl hydrocarbon receptor by structurally diverse exogenous and endogenous chemical[J]. Annu Rev Pharmacol Toxicol,2003,43:309-334.
[24] Nims RW,Lubet RA,Fox SD,et al. Comparative pharmaco-dynamics of CYP2B induction by DDT,DDE,and DDD in male rat liver and cultured rat hepatocytes[J]. J Toxicol Environ Health A,1998,53(6):455-477.
[25] Jeong HG,Kim JY. Effects of o,p'-DDT on the 2,3,7,8-tetrachlorodibenzo-p-dioxin-inducible CYP1A1 expression in murine Hepa-1c1c7 cells[J]. Food Chem Toxicol,2002, 40(11):1685-1692.
[26] ATSDR. Agency for toxic substances and diseases registry toxicological profile for DDT,DDE and DDD[R]. US Department of Health and Human Services,2002.
[27] Oliveira HH,Babin M,Garcia JR,et al. Complex metabolic interactions between benzo(a)pyrene and tributyltin in presence of dichlorodiphenyltrichloroethane in South American catfish Rhamdia quelen[J]. Ecotoxicol Environ Saf,2013,96:67-74.
[28] Lakshmi B,Ajith TA,Jose N,et al. Antimutagenic activity of methanolic extract of ganoderma lucidμM and its effect on hepatic damage caused by benzo[a]pyrene[J]. J Ethnophar-macol,2006,107(2):297-303.
[29] Cosan D,Basaran A,Gunes HV,et al. The effect of doxorubicin on rats that received toxic and carcinogenic benzo(a)pyrene [J]. Folia Histochem Cytobiol,2008,46(3):367-372.

Effects of benzo[a]pyrene and DDT subacute co-exposure on ALT, AST and γ-GT in mice

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