NNK诱发人支气管上皮细胞恶性转化及氧化损伤机理研究

doi:10.3969/j.issn.1004-616x.1999.04.008

Carcinogenesis, Teratogenesis & Mutagenesis ›› 1999, Vol. 11 ›› Issue (4): 184-188.doi: 10.3969/j.issn.1004-616x.1999.04.008

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NNK INDUCED HUMAN BRONCHIAL EPITHELIAL CELL LINE NEOPLASTIC TRANSFORMATION AND ITS MECHANISM OF OXIDATIVE DAMAGE

Zhihua Yang1 , Maoxiang Zhu1 , Yifeng Gong1 , Yong Xu2 , Hongyan Sun1

1 Instit ute of Radiation Medicine , Beijing 　100850 　2 Department of Pharmacology & Toxicology , School of Medicine , Indiana University , USA

Received:1999-01-16 Revised:1999-04-04 Online:1999-07-30 Published:1999-07-30

Abstract

Abstract: The HPV216 immortalized human bronchial epithelial cell line (BEP2D) was used to study NN K in2 duced cell t ransformation and it s mechanism of oxidative damage. The 5th passage BEP2D cells t reated with 500μg/ ml NN K(NN K2500 cells) could grow in meduum containing fetal calf serum. The 15th passage NN K500 cells increased significantly colony formation in soft agar. The 25th passage N K2500 cells had the ability to grow tumor in nude mice. The level of active oxygen species productions including hydrogen peroxides(H2O2) ,superoxide anions(O ÷2 ) and hydroxyl radicals (·OH) , and the contents of 2hydroxydeoxyguanosine (OH8dG) in NN K2t reated BEP2D cells , which maybe involved mechanism ofoxidative damage.

Key words: NN K, BEP2D cell line, Neoplastic t ransformation, Active oxygen species, DNA adduct

Zhihua Yang , Maoxiang Zhu , Yifeng Gong , Yong Xu , Hongyan Sun. NNK INDUCED HUMAN BRONCHIAL EPITHELIAL CELL LINE NEOPLASTIC TRANSFORMATION AND ITS MECHANISM OF OXIDATIVE DAMAGE[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 1999, 11(4): 184-188.

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NNK INDUCED HUMAN BRONCHIAL EPITHELIAL CELL LINE NEOPLASTIC TRANSFORMATION AND ITS MECHANISM OF OXIDATIVE DAMAGE

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