运动训练对尿源干细胞原代产量及早期生物学特性的影响

doi:10.3969/j.issn.1004-616x.2025.06.008

Abstract

Abstract: OBJECTIVE：To evaluate the impact of exercise training on primary yield and early biological quality of urine-derived stem cells (USCs) from a single volunteer and to determine feasibility of collecting samples at a fixed evening interval (20:00-21:00)，thereby providing guidance for non-invasive and standardized USCs isolation. METHODS：A self-controlled design was adopted. Urine samples were collected from the same volunteer at four time points-early morning (6:00-7:00)，forenoon (9:00-10:00)，afternoon (14:00-15:00) and evening (20:00-21:00)-to compare primary clone-forming efficiency and identify the optimal sampling period. Within this period，the volunteer first maintained normal daily activity for 7 days (rest group) and then performed a standardized run (2.8 km in 16-18 min) daily for another 7 days (exercise group)，with urine collected immediately post-exercise. USCs were isolated and cultured under identical protocols. Cellular morphology was assessed by morphological observation. Cell proliferation was assessed by MTT assay over 7 days to prepare growth curves. RT-qPCR was employed to measure mRNA levels of the pluripotency genes OCT4，C-MYC，SOX2 and NANOG，while Western blot was used to evaluate OCT4 and C-MYC protein expression. RESULTS：Evening collection (20:00-21:00) consistently yielded USC clones with the highest formation rate. Within this window，exercise-derived USCs displayed homogeneous morphology and lower senescence versus sedentary controls. The proliferation curve revealed a significantly higher D(570) on day 7 (P<0.01)，indicating sustained proliferative capacity. OCT4 and C-MYC mRNA levels were up-regulated (P<0.05 and P<0.01，respectively)，whereas SOX2 and NANOG mRNA levels remained unchanged (P>0.05). Consistently，OCT4 and C-MYC protein expression were markedly elevated (P<0.01). CONCLUSION：Evening urine collection (20:00-21:00) combined with exercise training significantly improved USC extraction efficiency and enhanced their early biological characteristics，offering a simple and feasible strategy for subsequent scalable expansion and clinical translation.

Key words: urine-derived stem cells, exercise training, primary culture, sampling time, cell proliferation, stemness maintenance

CLC Number:

R329.2+8

XU Jiaqi, WANG Fenglong, SUN Zhenxiao. Impact of exercise training on primary yield and biological quality of urine-derived stem cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2025, 37(6): 473-477,482.

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Impact of exercise training on primary yield and biological quality of urine-derived stem cells

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