Carcinogenesis, Teratogenesis & Mutagenesis ›› 2000, Vol. 12 ›› Issue (2): 84-086.doi: 10.3969/j.issn.1004-616x.2000.02.007

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QUANTIFICATION OF EARLY APOPTOSIS OF GASTRIC CARCINOMA WITH AV AND PI BY FLOW CYTOMETER

ZHOU Hong - ping1 , WANG Xu1 , ZHANG Nan - zheng2 , HUANG Jian3   

  1. 1. Instit ute of Oncology , Xuz hou Medical College , Xuz hou 221002 , China ; 2. Department of Oncology ,PL A 97 th Hospital , Xuz hou 221002 , China ; 3. Cent ral L aboratory , A f f ilited Hospital of Xuz hou Medical College , Xuz hou 221002 , China
  • Received:1999-06-21 Revised:1999-11-25 Online:2000-04-30 Published:2000-04-30

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Abstract: Purpose : Measuring the amounts of fluorescence by bivariate flow cytomet ry ( FCM) , we examined early stage of apoptosis in 6 vicious gast ric tissues and corresponding normal ones with a double staining method of Annexin V(AV) and propidiom iodide (PI) . Methods : From 6 vicious and 6 normal gast ric tissues of 1cm3 ,single2cell suspension were preparated and stained with fluorescein isothiocyanate ( FITC)2labelled AV and PI ,and AV binding was assessed using bivariate FCM. Results : The separated cells incubated with AV and PI enabled three distinct populations of cells(apoptotic , necrotic and living cells) to be identified and sorted by FCM.Comparing normal and vicious tissues , differences were great among the three populations of cells. Conclusion :The AV/ PI bivariate FCM allows the possibility of early phase of apoptosis in early gast ric carcinoma , which offers a method to research clinically gast ric carcinoma.

Key words: Annexin V, propidiom iodide, gast ric carcinoma, early stage of apoptosis, quantification

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