Abstract: BACKGROUND ＆ AIM: To investigate the difference of gene expression in fetal lung and normal tissue. MATERIAL AND METHODS: The cDNA retro－transcribed from the mRNA which was purified from the total RNA extracted from fetal lung tissue and normal lung tissue were labeled with Cy5 and Cy3 fluorescence as the probes. Then we hybrided the probes hybrided were with the whole gene chip which was later scanned by ScanArray4000 fluorescence scanner to acquire the signal image and analyzed with software. RESULTS: 317 genes expressed in fetal lung and normal tissue were screened, among these genes,up-and-down-regulated genes were 193 and 124 respectively. CONCLUSION : There is difference in gene between fetal two Lung and normal tissues. Human Lung development is affected by environmental conditions.

Key words: fetal lung, normal tissue, gene expression profile