乙酸镍诱导的人支气管上皮细胞系转化细胞基因组不稳定性分析

doi:10.3969/j.issn.1004-616x.2005.01.008

Carcinogenesis, Teratogenesis & Mutagenesis ›› 2005, Vol. 17 ›› Issue (1): 25-26.doi: 10.3969/j.issn.1004-616x.2005.01.008

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Genomic Instability in Transformed 16HBE Cells Induced by Nickel Acetate

CHEN Chuan-de; WU Zhong-liang; CHEN Jia-kun

Center for Disease Control and Prevention of Luohu district, Shenzhen,Shenzhen 518020, China

Received:2004-03-16 Revised:2004-05-08 Online:2005-01-30 Published:2005-01-30
Contact: CHEN Chuan-de

Abstract

Abstract: BACKGROUND ＆ AIM：To detect the genomic instability in the 16HBE cells induced by nickel acetate. MATERIAL AND METHODS： Analysing the genomic instability in the 16HBE cells induced by nickel acetate by random amplified polymorophic DNA(RAPD).RESULTS：Compared with the negative control cells, 16HBE cells induced by nickel acetate have special PCR bands, indicating genomic instability. But the bands amplified by forth and seventh primes have no difference. CONCLUSION： Crystalline nickel sulfide could induce genomic instability in the 16HBE cells induced by nickel acetate.

Key words: nickel, genomic instability, RAPD

CHEN Chuan-de, WU Zhong-liang, CHEN Jia-kun. Genomic Instability in Transformed 16HBE Cells Induced by Nickel Acetate[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2005, 17(1): 25-26.

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Genomic Instability in Transformed 16HBE Cells Induced by Nickel Acetate

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