Abstract: BACKGROUND＆AIM: To investigate the effects of Prescription of Traditional Chinese Medicine-1(PTCM-1) on the proliferation and the cell cycle change arrest of human gastric carcinoma cell line SGC-7901 in vitro. MATERIAL AND METHODS: The proliferation of SGC-7901 cells was measured by 3-[4,5-dimethylthiazol-2-y1]-2,5 dipheny1 tetrazolium bromide(MTT) colorimetric assay. The cell cycle phase was analysised by flow cytometry. RESULTS: PTCM-1 inhibited the proliferation of SGC-7901 cells in dose-dependent manner and in time-dependent manner from 0 h to 72 h. After treated by PTCM-1 for 48 h, the SGC-7901 cells were accumulated in G2＋M phase in the cell cycle, and the ratio of cells in G0/G1, S phase was decreased. CONCLUSION: PTCM-1 can inhibit proliferation of SGC-7901 cells. It interferences into the cell cycle may be one of mechinism of its antitumor activity.

Key words: prescription of traditional Chinese medicine, human gastric carcinoma cell;proliferation;cell cycle