Abstract: Purpose : This paper studies the relationships between Heated Cooking Oil Vapors (HCOV) and Allium Sativum Oil (ASO) in mutagenesis. Methods : we detected p53 and p21ras protein expression in the V79 cells treated by HCOV and ASO with immunocytochemical techneques. Results : The result s showed that the p53 and p21ras protein expression was significantly increased in HCOV groups compared with the cont rol group ( P < 0.01) , and a dose2dependent relationship was observed , while the ASO(50μg/ ml) could decreased the p53 and p21ras protein expression in the V79 cells t reated by HCOV compared with the cont rol group ( P < 0. 05 or P < 0.01) . Conclusion : The result s indicated that the ASO could prevent f rom mutagenic activity of the HCOV.

Key words: heated cooking oil vapors, ailium sativum oil, p53, p21ras, immunocytochemistry