电离辐射对人乳腺癌MCF7 细胞系hR24L 基因表达的影响

doi:10.3969/j.issn.1004-616x.2001.02.001

Abstract

Abstract: Purpose : To study the effect of radiation on the expression of human DNA repair gene hR24L. Methods : We amplified it s open reading f rame f rom human breast cancer MCF7 cells by RT2PCR , and had its sequence checked , irradiated the cells with gamma2ray at a total dose of 5 Gy followed by incubating the cells for different time , collecting cells , isolating the total RNA , analysed hR24L2mRNA by Northern bloting. results :The expression of hR24L2mRNA began to increase at about one hour after irradiation and reached the maximum at about two hours after irradiation. Conclusion : The expression of hR24L gene is damage2inducible , this expression can repair the damage caused by gamma2ray irradiation.

Key words: hR24L, gene, DNA repair, irradiation

CLC Number:

Q754

LI Ping-fa, ZHU Ying-bao, HAN Yun, JIA Ting-zhen, TONG Tan-jun. THE EFFECT OF IRRADIATION ON THE EXPRESSION OF HUMAN DNA REPAIR GENE HR24L[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2001, 13(2): 67-069.

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THE EFFECT OF IRRADIATION ON THE EXPRESSION OF HUMAN DNA REPAIR GENE HR24L

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