关键词: 弱精症, 精子, 基因芯片, mRNA, 表达谱

Abstract: BACKGROUND AND AIM: To construct and analyze the gene-expression profiles in the sperms of asthenospermic patients. MATERIALS AND METHODS: 30 semen samples from asthenospermia patients and 20 semen samples from healthy normal adult men were collected; and total RNAs extracted to produce cDNAs probes. Hybridization with Phalanx OneArrayTM contained 30 968 probes was carried out after the labeled cDNAs were purified by PCR Product Purification Kit. RESULTS: Among the 30 968 probes; 394 genes were up-regulated; 437 genes were down-regulated; the others showed no different expressions. Functional cluster displayed that response to external stimulus; defense; stress and inflammation were the most important reasons leading to asthenospermia; and protease inhibitor could also lead to asthenospermia. Furthermore; genes associated with spermatogenesis and negative regulation of apoptosis were very important to maintain high sperm motility. CONCLUSION: Construction of gene-expression profiles in the sperms of asthenospermic patients could help to analyze the molecular mechanism of sperm motility and to study the etiopathogenisis of asthenospermia.

Key words: asthenospermia, sperm, microarrays, gene-expression profiles