精子与肿瘤细胞融合模型的生物学特性研究

doi:10.3969/j.issn.1004-616x.2018.03.011

癌变·畸变·突变 ›› 2018, Vol. 30 ›› Issue (3): 221-225.doi: 10.3969/j.issn.1004-616x.2018.03.011

精子与肿瘤细胞融合模型的生物学特性研究

陈强^1,2, 靳广毅¹, 林桂淼¹, 刘好¹, 许改霞², 王晓梅¹

1. 深圳大学医学院生理学教研室, 广东深圳 518060;
2. 深圳大学光电子器件与系统教育部重点实验室, 广东深圳 518060

收稿日期:2017-12-11 修回日期:2018-03-06 出版日期:2018-05-30 发布日期:2018-05-30
通讯作者: 王晓梅,E-mail:xmwang@szu.edu.cn E-mail:xmwang@szu.edu.cn
作者简介:陈强,E-mail:xy198033@sina.com。
基金资助:
国家科技部国家重点研发计划项目（2016YFC0904601）；深圳市基础研究项目（JCYJ20170303160906960）；深圳市国际合作项目（GJHZ20170313111237888）；深圳市科创委布局项目（基20160084）

Biological characteristics of a sperm-tumor cell fusion model

CHEN Qiang^1,2, JIN Guangyi¹, LIN Guimiao¹, LIU Hao¹, XU Gaixia², WANG Xiaomei¹

1. Department of Physiology, School of Medicine, Shenzhen University, Shenzhen 518060;
2. Key Laboratory of Optoelectronics Devices and Systems of Ministry of Education, College of Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, Guangdong, China

Received:2017-12-11 Revised:2018-03-06 Online:2018-05-30 Published:2018-05-30

摘要/Abstract

摘要： 目的：探讨精子与肿瘤细胞融合模型的生物学特性。方法：采用体外共培养对精子与肿瘤细胞融合模型的时效性、细胞接种密度依赖性及细胞类型特异性进行分析。采用显微操作及单克隆培养法获得小鼠精子与HeLa细胞融合的嵌合型单克隆细胞株，并对其进行生物学鉴定。采用体外扩增Y染色体特异基因TSPY的方法鉴别宫颈癌石蜡组织中的嵌合型肿瘤细胞。结果：精子与肿瘤细胞在体外的融合通常发生于共培养后的1.5 h，精子与肿瘤细胞融合率随细胞接种密度的升高而降低，精子与腺癌来源的肿瘤细胞具有更高的细胞融合率。在20例宫颈癌蜡块标本中我们发现1例存在嵌合型肿瘤细胞，而且其TSPY基因存在1处有义突变。结论：精子与肿瘤细胞融合存在时效性、细胞接种密度依赖性及细胞类型特异性特征。在宫颈癌标本中存在嵌合型肿瘤细胞。

关键词: 精子, 肿瘤细胞, 细胞融合, 嵌合细胞

Abstract: OBJECTIVE: To explore the biological characteristics of sperm-tumor cell fusion model. METHODS: A co-cultivation in vitro methods was used to study the time-effectiveness,cell inoculation density dependence and cell type specificity of the fusion model. The chimeric sperm-HeLa monoclonal cells were obtained by using micromanipulation and monoclonal culture method,which was then identified by using PCR test. The chimeric tumor cells in the cervical cancer paraffin were detected by amplification Y chromosome specific gene TSPY. RESULTS: Fusion between the sperm and tumor cells generally occurred in 1.5 hours after co-cultivation,the fusion rates was inversely proportional to the cell inoculation density,and the tumor cells who derived from an adenocarcinoma had a higher rate of cell fusion than other tumor cells and normal controls. One chimeric tumor tissue was observed in 20 cases of cervical cancer specimens,and one righteous mutation was found in TSPY gene. CONCLUSION: Cell fusion between the sperm and tumor cells displayed time-effectiveness,cell inoculation density dependence and cell type specificity. The chimeric tumor cells could be found in cervical cancer specimens.

Key words: sperm, tumor cells, cell fusion, chimeric cells

中图分类号:

Q279
Q944.47

陈强, 靳广毅, 林桂淼, 刘好, 许改霞, 王晓梅. 精子与肿瘤细胞融合模型的生物学特性研究[J]. 癌变·畸变·突变, 2018, 30(3): 221-225.

CHEN Qiang, JIN Guangyi, LIN Guimiao, LIU Hao, XU Gaixia, WANG Xiaomei. Biological characteristics of a sperm-tumor cell fusion model[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(3): 221-225.

参考文献

[1] SAWICKI W,KOPROWSKI H. Fusion of rabbit spermatozoa with somatic cells cultivated in vitro[J]. Exp Cell Res,1971,66(1):145-151.
[2] BENDICH A,BORENFREUND E,STERNBERG S S. Penetration of somatic mammalian cells by sperm[J]. Science,1974,183(4127):857-859.
[3] HIGGINS P J,BORENFREUND E,BENDICH A. Appearance of foetal antigens in somatic cells after interaction with heterologous sperm[J]. Nature,1975,257(5526):488-489.
[4] VAN MEEL F C,PEARSON P L. Do human spermatozoa reactivate in the cytoplasm of somatic cells?[J]. J Cell Sci,1979,35:105-122.
[5] KHOSROTEHRANI K,JOHNSON K L,CHA D H,et al. Transfer of fetal cells with multilineage potential to maternal tissue[J]. JAMA,2004,292(1):75-80.
[6] MIKHAIL M A,M'HAMDI H,WELSH J,et al. High frequency of fetal cells within a primitive stem cell population in maternal blood[J]. Hum Reprod,2008,23(4):928-933.
[7] KHOSROTEHRANI K,BIANCHI D W. Multi-lineage potential of fetal cells in maternal tissue:a legacy in reverse[J]. J Cell Sci,2005,118(Sup 8):1559-1563.
[8] KHOSROTEHRANI K,REYES R R,JOHNSON K L,et al. Fetal cells participate over time in the response to specific types of murine maternal hepatic injury[J]. Hum Reprod,2007,22(3):654-661.
[9] 陈强,靳广毅,林桂森,等. 精子与肿瘤细胞融合模型的建立[J]. 癌变·畸变·突变,2018,29(3):144-149.
[10] O'DONOGHUE K,SULTAN H A,AL-ALLAF F A,et al. Microchimeric fetal cells cluster at sites of tissue injury in lung decades after pregnancy[J]. Reprod Biomed Online,2008,16(3):382-390.
[11] CIRELLO V,RECALCATI M P,MUZZA M,et al. Fetal cell microchimerism in papillary thyroid cancer:a possible role in tumor damage and tissue repair[J]. Cancer Res,2008,68(20):8482-8488.
[12] DOBRZYŃSKA I,SZACHOWICZ-PETELSKA B,SULKOWSKI S,et al. Changes in electric charge and phospholipids composition in human colorectal cancer cells[J]. Mol Cell Biochem,2005,276(1/2):113-119.
[13] SZACHOWICZ-PETELSKA B,DOBRZYŃSKA I,FIGAS-ZEWSKI Z,et al. Changes in physicochemical properties of human large intestine tumor cells membrane[J]. Mol Cell Biochem,2002,238(1/2):41-47.
[14] HOLLINGSWORTH M A,SWANSON B J. Mucins in cancer:protection and control of the cell surface[J]. Nat Rev Cancer,2004,4(1):45-60.
[15] DOBRZYŃSKA I,SZACHOWICZ-PETELSKA B,DAREWICZ B,et al. Characterization of human bladder cell membrane during cancer transformation[J]. J Membr Biol,2015,248(2):301-307.
[16] CABODI S,DEL PILAR CAMACHO-LEAL M,DI STEFANO P,et al. Integrin signaling adaptors:not only figurants in the cancer story[J]. Nat Rev Cancer,2010,10(12):858-870.
[17] MATTIOLI M,GLORIA A,MAURO A,et al. Fusion as the result of sperm-somatic cell interaction[J]. Reproduction,2009,138(4):679-687.

精子与肿瘤细胞融合模型的生物学特性研究

Biological characteristics of a sperm-tumor cell fusion model

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