多核细胞检测法的方法学探讨

doi:10.3969/j.issn.1004-616x.1998.01.019

癌变·畸变·突变 ›› 1998, Vol. 10 ›› Issue (1): 57-061.doi: 10.3969/j.issn.1004-616x.1998.01.019

多核细胞检测法的方法学探讨

邬洪梁1 　朱　巍1 　郑斯英1 　高锦声2

1苏州医学院放射生物学教研室　2 生物学教研室　苏州215007

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1998-01-30 发布日期:1998-01-30

THE METHODOLOGY ON THE MULTINUCLEATED CELL ASSAY

Wu Hongliang1 , Zhu Wei1 , Zheng Siying2 , Gao J insheng1

1 Department of radiation biology , 2 Department of biology , S uz hou Medical College , S uz hou 　 215007

Received:1900-01-01 Revised:1900-01-01 Online:1998-01-30 Published:1998-01-30

摘要/Abstract

摘要： 本文应用L9 (3) 4 正交试验设计对影响多核细胞检测法的三个主要因素进行了探讨。结果表明:6 -TG终浓度为2 ×10 - 5M , Cyt - B 终浓度为6mg/ l , 72 小时收获细胞的组合为最佳实验搭配方案。同时对其它影响因素也作了研究。

关键词: 多核细胞检测法, 方法学, 正交试验, hprt 基因位点变异频率

Abstract: Three main factors influcing the multinucleated cell assay were evaluated with the L9 (3) 4 orthogonal design. The result was the best when the 6 - TG final density was 2 ×10 - 5M , the Cyt - B final density was 6mg/ l , and the culture time was 72 hours. Meanwhile the other factors were also studied.

Key words: the multinucleated cell assay, methodology, orthogonal design, hprt gene locus variant f requency

邬洪梁　朱　巍　郑斯英　高锦声. 多核细胞检测法的方法学探讨[J]. 癌变·畸变·突变, 1998, 10(1): 57-061.

Wu Hongliang , Zhu Wei , Zheng Siying , Gao J insheng. THE METHODOLOGY ON THE MULTINUCLEATED CELL ASSAY[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 1998, 10(1): 57-061.

多核细胞检测法的方法学探讨

THE METHODOLOGY ON THE MULTINUCLEATED CELL ASSAY

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