关键词: 微团培养, 胚胎中脑细胞, 大鼠, 五氧化二钒, 发育毒性

Abstract: Vanadium is a proven animal teratogen which can induce dilation of latermal vent ricles and skeletal abnormal in rat , although the mechanism of it s developmental toxicity is unknown. In this paper , the micromass rat embryo midbrain cell culture was used in a affort to determine potential mechanisms by which Vanadium Pentoxide (V2O5) may exert it s teratogenic effect . In vit ro test , a dose2dependent inhibition of differentiation and proliferation of midbrain cells were observed. The 50 % - inhibitory concent ration ( IC50) for cell differntiation and cell survial or proliferation 50 % the cont rol value ( IC50) are 6. 8 and 9. 1μmol/ L , respcectively. In vivo/ in vit ro test , V2O5 could inhibit cell differentiation at 2. 0 mg/ kg body weight , but inhibit cell surival or proliferation at 5. 0mg/ kg. According to class standard of Renault , el al (1989) , V2O5 may be a specific inhibitor of embryonic midbrain cell.

Key words: Micromass Culture, Embryo midbrain cell, Rat, Vandium Pentoxide, Developmental toxicity