癌变·畸变·突变 ›› 1998, Vol. 10 ›› Issue (4): 193-197.doi: 10.3969/j.issn.1004-616x.1998.04.001

• 论著 •    下一篇

丙肝病毒ns3 基因转基因小鼠的产生

张树忠1 　李建秀1 　戚中田2 　胡以平1   

  1. 1第二军医大学基础部生物学教研室　2第二军医大学基础部微生物学教研室　上海　200433
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1998-07-30 发布日期:1998-07-30

THE GENERATION OF TRANSGENIC MICE HARBOURING NONSTRUCTURAL 3 GENE OF HEPATITIS C VIRUS

Zhang Shuzhong1 , Li J ianxiu1 , Hu Yiping1 and Qi Zhongtian2   

  1. 1Department of Biology , Second Military Medical University , 2Department of Microbiology , Second Military Medical University , S hanghai 200433
  • Received:1900-01-01 Revised:1900-01-01 Online:1998-07-30 Published:1998-07-30

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摘要： 通过受精卵原核显微注射的途径,产生带有可诱导型丙肝病毒非结构蛋白3 基因( non2structural 3gene) 的重组载体的转基因小鼠。采用PCR 和基因组Southern2blot 杂交方法,在21 只新生鼠中筛选到3 只建立者小鼠。其中2 只与非转基因小鼠进行交配,分别得到8 只和6 只F1 代小鼠。进而对其基因组DNA 进行检测,发现其中4 只为转基因阳性个体。这些小鼠的获得,为ns3 转基因品系的建立和NS3 蛋白的生物学作用研究准备了条件。

关键词: 非结构蛋白3 基因, 丙肝病毒, 转基因小鼠

Abstract: To generate t ransgenic mice , the recombinant plasmids inserted the ns3 gene of hepatitis C virus were int roduced into the male pronuclei of mouse fertilizer eggs by microinjection. By using polymerase chain reaction(PCR) and Southern2blot hybridization method we obtained three mice (Founders) and four mice ( F1) harbouring the ns3 gene among 21 off springs born by the pseudopregnant mice and 14 individuals generated f rom the Founders mated with other non2t ransgenic mice , respectively. These t ransgenic mice would be helpful for the development of ns3 t ransgenic mice line and the study for the biological function of NS3 protein in vivo.

Key words: Non-structural 3 gene, Hepatitis C Virus, Transgenic Mice

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