上海正常人群中谷胱甘肽S - 转移酶T1 基因纯合缺损

doi:10.3969/j.issn.1004-616x.1998.06.004

癌变·畸变·突变 ›› 1998, Vol. 10 ›› Issue (6): 345-348.doi: 10.3969/j.issn.1004-616x.1998.06.004

上海正常人群中谷胱甘肽S - 转移酶T1 基因纯合缺损

林国芳　谭靖伟　沈建华

中国科学院上海昆虫研究所中德毒理学实验室　上海　200025

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1998-11-30 发布日期:1998-11-30

HOMOZYGOUS DEFICIENCY OF HUMAN GLUTATHIONE STRANSFERASE T1 GENE IN NORMAL POPULATION IN SHANGHAI

Lin Guofang ,Tan J ingwei ,Shen J ianhua

S ino2German L aboratory of Toxicology in S hanghai ( DCL T) , Shanghai Institute of Entomology , Academia Sinica , Shanghai 　200025

Received:1900-01-01 Revised:1900-01-01 Online:1998-11-30 Published:1998-11-30

摘要/Abstract

摘要： 人类谷胱甘肽S - 转移酶是一类重要的Ⅱ相反应代谢酶,它不仅与环境有害物质的减毒代谢有关,有时也与活化代谢过程有关,在胞浆谷胱甘肽S - 转移酶中GSTT1 表现的遗传多态性主要是由相关编码基因的缺失造成的。本文通过由人β- 珠蛋白基因片段作内标控制对人血有核细胞DNA 进行GSTT1 等位基因专一(Allele - specific) PCR 扩增的方法,对226 名上海市健康常住居民进行GSTT1 基因型检测,结果表明5013 %(114/ 226) 的检测对象为GSTT1 基因纯合子缺损( GSTT10/ 0 基因型) ,上述结果显著高于世界各地高加索人种集团的报道值(11 - 17 %) 以及美国黑人人群的相应数值(24 %) 。

关键词: 谷胱甘肽S - 转移酶T1, 正常人群, 基因缺损

Abstract: Human glutathione S2t ransferase ( GSTs) is considered as an important group of phase reaction enzymes involved not only in the detoxification metabolism ,in some cases , also in the bioactivation process of various types of environmental hazard chemicals. Among the glutathione S2t ransferases known so far , GSTT1 displays genetic polymorphism due to gene deletion. GSTT1 was genotyped in 226 healthy adults in Shanghai (permanent resident s) by an internal control allele2specific PCR amplification of blood nucleated cell DNA. The homozygous deletion of gene ( GSTT10/ 0 genotype) was identified in 50. 3 %(114/ 226) of the individuals tested. The deficiency frequency in normal population in Shanghai is higher than in normal Caucasian population (11 - 17 %) and among Af rican Americans (24 %) significantly.

Key words: glutathione S-transferase T1, Normal population, Gene deficiency

林国芳　谭靖伟　沈建华. 上海正常人群中谷胱甘肽S - 转移酶T1 基因纯合缺损[J]. 癌变·畸变·突变, 1998, 10(6): 345-348.

Lin Guofang , Tan J ingwei , Shen J ianhua. HOMOZYGOUS DEFICIENCY OF HUMAN GLUTATHIONE STRANSFERASE T1 GENE IN NORMAL POPULATION IN SHANGHAI[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 1998, 10(6): 345-348.

[1]	梁子卿 , 宋忠魁 , 夏晓玲 , 黄云超 , 镡　颖 , 汪　旭 . 云南籍几个正常人群间GSTM1 基因多态性的比较研究[J]. 癌变·畸变·突变, 2002, 14(1): 22-024.
[2]	林国芳, 马晴雯, 查永林, 娄克俭, 陈纪刚, 沈建华. 上海“本地人”正常人群GSTT1 和GSTM1 基因型多态性研究[J]. 癌变·畸变·突变, 2001, 13(1): 10-012.

上海正常人群中谷胱甘肽S - 转移酶T1 基因纯合缺损

HOMOZYGOUS DEFICIENCY OF HUMAN GLUTATHIONE STRANSFERASE T1 GENE IN NORMAL POPULATION IN SHANGHAI

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 2

编辑推荐

Metrics

本文评价