钙调素拮抗剂对镉接触小鼠脑突触小体蛋白质合成的影响

doi:10.3969/j.issn.1004-616x.1999.06.017

癌变·畸变·突变 ›› 1999, Vol. 11 ›› Issue (6): 337-339.doi: 10.3969/j.issn.1004-616x.1999.06.017

钙调素拮抗剂对镉接触小鼠脑突触小体蛋白质合成的影响

唐玲芳　陈砚朦　张珍玲　宋　玲　杨永年　冯致英

南京医科大学　卫生毒理学教研室　南京　210029

收稿日期:1999-07-26 修回日期:1999-09-01 出版日期:1999-11-30 发布日期:1999-11-30

INFULENCES OF CALMODULIN ANTAGONIST ON HE EFFECTS OF CADMIUM IN BRAIN SYNAPTOSOME PROTEIN BIOSYNTHESIS OF MICE

Tang lingfang , Chen Yanmeng , Zhang Zhenling , SongLing , Yang Yongnian , Feng Zhiying

Department of Toxicology , Nanjing Medical University , Nanjing 　210029

Received:1999-07-26 Revised:1999-09-01 Online:1999-11-30 Published:1999-11-30

摘要/Abstract

摘要： 摘要　采用体外高速梯度离心分离提取的小鼠脑突触小体,观察钙调素拮抗剂对镉接触脑突触小体中3H - 亮氨酸掺入蛋白质合成的影响。结果表明镉(Cd) 抑制3H - 亮氨酸掺入突触小体蛋白质合成,3H - 亮氨酸掺入量与镉浓度呈负相关。这一作用可被钙调素拮抗剂氯丙嗪一定程度上拮抗。提示钙调素参与镉的毒性作用机制。

关键词: 关键词　脑突触小体, 蛋白质合成, 钙调素拮抗剂

Abstract: Abstract 　The isolated mice brain synaptosome was measured in vitro. The experiment explored protein biosynthesis by 3H - Leu in2 corporation technique , meanwhile , the influence of Calmodulin(CaM) inhibitor Chlorpromazine (CPZ) on this toxicity was investigated as well. The result indicated that Cd prevented 3H - Leu from incorporating into synaptosome , in which the concentration of Cd and the amount incorporated showed negative correlation and this toxic effect may be antagonized by CaM inibitor CPZ to some extend. It is concluded that CaM may be involved in the mechanism of Cd toxicity in brain synaptosome.

Key words: Key words 　Brain Synaptosome, Protein Biosynthesis, Calmodulin Inhibitor

唐玲芳　陈砚朦　张珍玲　宋　玲　杨永年　冯致英. 钙调素拮抗剂对镉接触小鼠脑突触小体蛋白质合成的影响[J]. 癌变·畸变·突变, 1999, 11(6): 337-339.

Tang lingfang , Chen Yanmeng , Zhang Zhenling , SongLing , Yang Yongnian , Feng Zhiying. INFULENCES OF CALMODULIN ANTAGONIST ON HE EFFECTS OF CADMIUM IN BRAIN SYNAPTOSOME PROTEIN BIOSYNTHESIS OF MICE[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 1999, 11(6): 337-339.

钙调素拮抗剂对镉接触小鼠脑突触小体蛋白质合成的影响

INFULENCES OF CALMODULIN ANTAGONIST ON HE EFFECTS OF CADMIUM IN BRAIN SYNAPTOSOME PROTEIN BIOSYNTHESIS OF MICE

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