关键词: 肿瘤原代细胞, 体外培养, 药物敏感性, MTT 比色法

Abstract: Purpose : In order to observe the effect of chemotherapeutic drugs and Zheng's plant - protein on 100 case human solid tumor cells and to obtain information of tumor cell growing and of select sensitive chemotherapeutic drugs. Methods : The activity of sample tumor cell in primary culture was detemined with MTT colorimet ry. Results and Conclusion : The proliferation of 30 % sample tumor was unwell , but the other 70 % was well.Result s showed high sensitivity on sample tumor cell are cisplatin , carboplatinum , 5 - Fluorouracil , mitomycin and bleomycin. The proliferation of the tumor cells was promoted by stimulation of Zheng’s plant - protein .The proliferation of a few sample tumor cells was promoted by incristine , adriamycin and vepeside also.

Key words: Tumor cell, primary culture assay in vitro, drug sensitivity, MTT colorimetry